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Analysis of microRNA expression profiles in porcine PBMCs after LPS stimulation.
Innate Immunity ( IF 2.8 ) Pub Date : 2020-01-22 , DOI: 10.1177/1753425920901560
Jing Zhang 1 , Xin Xu 1 , Xingfa Huang 2 , Huiling Zhu 1 , Hongbo Chen 1 , Wenjun Wang 2 , Yulan Liu 1
Affiliation  

In the present study, we used microRNA (miRNA) sequencing to discover and explore the expression profiles of known and novel miRNAs in 1000 ng/ml LPS stimulated for 8 h vis-à-vis non-stimulated (i.e. control) PBMCs isolated from the blood of healthy pigs. A total of 291 known miRNAs were bio-computationally identified in porcine PBMCs, and 228 novel miRNAs (not enlisted in the swine mirBase) were identified. Among these miRNAs, ssc-miR-148a-3p, ssc-let-7g, ssc-let-7f, 3_8760, ssc-miR-26a, ssc-miR-451, ssc-miR-21, ssc-miR-30d, ssc-miR-99a and ssc-miR-103 were the top 10 most abundant miRNAs in porcine PBMCs. Through miRNA differential analysis combined with quantitative PCR, we found the expressions of ssc-miR-122, ssc-miR-129b, ssc-miR-17-5p and ssc-miR-152 were significantly changed in porcine PBMCs after LPS stimulation. Furthermore, targets prediction and function analysis indicated a significant enrichment in gene ontology functional categories related to diseases, immunity and inflammation. In conclusion, this study on profiling of miRNAs expressed in LPS-stimulated PBMCs provides an important reference point for future studies on regulatory roles of miRNAs in porcine immune system.



中文翻译:

LPS刺激后猪PBMC中microRNA表达谱的分析。

在本研究中,我们使用microRNA(miRNA)测序来发现和探索已知和新型miRNA在1000 ng / ml LPS中刺激8 h的表达谱,相对于未刺激的(即对照)PBMC分离的PBMC健康猪的血液。在猪PBMC中通过生物计算鉴定出总共291种已知miRNA,并鉴定出228种新miRNA(未在猪mirBase中征募)。在这些miRNA中,ssc-miR-148a-3p,ssc-let-7g,ssc-let-7f,3_8760,ssc-miR-26a,ssc-miR-451,ssc-miR-21,ssc-miR-30d, ssc-miR-99a和ssc-miR-103是猪PBMC中最丰富的前十大miRNA。通过miRNA差异分析与定量PCR相结合,我们发现LPS刺激后猪PBMC中ssc-miR-122,ssc-miR-129b,ssc-miR-17-5p和ssc-miR-152的表达发生了显着变化。此外,目标预测和功能分析表明,与疾病,免疫力和炎症相关的基因本体功能类别显着丰富。总之,这项关于在LPS刺激的PBMC中表达的miRNA谱的研究为今后研究miRNA在猪免疫系统中的调节作用提供了重要的参考点。

更新日期:2020-01-22
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