In the present study, we used microRNA (miRNA) sequencing to discover and explore the expression profiles of known and novel miRNAs in 1000 ng/ml LPS stimulated for 8 h vis-à-vis non-stimulated (i.e. control) PBMCs isolated from the blood of healthy pigs. A total of 291 known miRNAs were bio-computationally identified in porcine PBMCs, and 228 novel miRNAs (not enlisted in the swine mirBase) were identified. Among these miRNAs, ssc-miR-148a-3p, ssc-let-7g, ssc-let-7f, 3_8760, ssc-miR-26a, ssc-miR-451, ssc-miR-21, ssc-miR-30d, ssc-miR-99a and ssc-miR-103 were the top 10 most abundant miRNAs in porcine PBMCs. Through miRNA differential analysis combined with quantitative PCR, we found the expressions of ssc-miR-122, ssc-miR-129b, ssc-miR-17-5p and ssc-miR-152 were significantly changed in porcine PBMCs after LPS stimulation. Furthermore, targets prediction and function analysis indicated a significant enrichment in gene ontology functional categories related to diseases, immunity and inflammation. In conclusion, this study on profiling of miRNAs expressed in LPS-stimulated PBMCs provides an important reference point for future studies on regulatory roles of miRNAs in porcine immune system.

在本研究中，我们使用microRNA（miRNA）测序来发现和探索已知和新型miRNA在1000 ng / ml LPS中刺激8 h的表达谱，相对于未刺激的（即对照）PBMC分离的PBMC健康猪的血液。在猪PBMC中通过生物计算鉴定出总共291种已知miRNA，并鉴定出228种新miRNA（未在猪mirBase中征募）。在这些miRNA中，ssc-miR-148a-3p，ssc-let-7g，ssc-let-7f，3_8760，ssc-miR-26a，ssc-miR-451，ssc-miR-21，ssc-miR-30d， ssc-miR-99a和ssc-miR-103是猪PBMC中最丰富的前十大miRNA。通过miRNA差异分析与定量PCR相结合，我们发现LPS刺激后猪PBMC中ssc-miR-122，ssc-miR-129b，ssc-miR-17-5p和ssc-miR-152的表达发生了显着变化。此外，目标预测和功能分析表明，与疾病，免疫力和炎症相关的基因本体功能类别显着丰富。总之，这项关于在LPS刺激的PBMC中表达的miRNA谱的研究为今后研究miRNA在猪免疫系统中的调节作用提供了重要的参考点。