BACKGROUND The pellicle, the acellular organic material deposited on the surface of tooth enamel, has been thought to be derived from saliva. In this study, protein compositions of the pellicle, gingival crevicular fluid, and saliva collected from healthy adults were compared to elucidate the origin of pellicle proteins. RESULTS The pellicle, gingival crevicular fluid, and saliva from the parotid gland or mixed gland were collected; subsequently, protein expression in samples from the respective individual was compared by SDS-PAGE and mass spectrometry. Following SDS-PAGE, proteins in the major bands were identified by mass spectrometry. The band pattern of pellicle proteins appeared different from those of gingival crevicular fluid, or saliva samples. Using mass spectrometry, 13 proteins in these samples were identified. The relative abundance of the proteins was quantitatively analyzed using mass spectrometry coupled with stable isotope labeling and by western blot. Cystatin S and α-amylase detected in pellicle were enriched in saliva samples, but not in gingival crevicular fluid, by western blot, and their abundance ratios were high in saliva and low in gingival crevicular fluid when analyzed by stable isotope labeling. Serotransferrin, however, was found only in the pellicle and gingival crevicular fluid by western blot and its abundance ratio was low in saliva. CONCLUSIONS Our study revealed that the gingival crevicular fluid appears to contribute to pellicle formation in addition to saliva.

中文翻译：

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防护膜，龈沟液和唾液的蛋白质谱比较：防护膜蛋白的可能来源。

背景技术防护膜是沉积在牙釉质表面上的无细胞有机材料，被认为是源自唾液。在这项研究中，比较了从健康成年人身上收集的防护膜，龈沟液和唾液的蛋白质组成，以阐明防护膜蛋白质的来源。结果收集了腮腺或混合腺的薄膜，龈沟液和唾液。随后，通过SDS-PAGE和质谱比较各个个体样品中的蛋白质表达。在SDS-PAGE之后，通过质谱鉴定主要谱带中的蛋白质。防护膜蛋白的条带模式似乎与龈沟液或唾液样品的条带模式不同。使用质谱法鉴定了这些样品中的13种蛋白质。蛋白质的相对丰度通过质谱联用稳定同位素标记和Western blot进行定量分析。通过western印迹，在薄膜中检测到的胱抑素S和α-淀粉酶在唾液样品中富集，而在龈沟液中则没有，在通过稳定同位素标记进行分析时，唾液中的丰度比高，而在龈沟液中的丰度比低。然而，通过western印迹仅在防护膜和龈沟液中发现了血清转铁蛋白，其唾液中的丰度比很低。结论我们的研究表明，除了唾液外，龈沟液似乎也有助于形成小薄膜。通过western印迹，在薄膜中检测到的胱抑素S和α-淀粉酶在唾液样品中富集，而在龈沟液中则没有，在通过稳定同位素标记进行分析时，唾液中的丰度比高，而在龈沟液中的丰度比低。然而，通过western印迹仅在防护膜和龈沟液中发现了血清转铁蛋白，其唾液中的丰度比很低。结论我们的研究表明，除了唾液外，龈沟液似乎也有助于形成小薄膜。通过western印迹，在薄膜中检测到的胱抑素S和α-淀粉酶在唾液样品中富集，而在龈沟液中则没有，在通过稳定同位素标记进行分析时，唾液中的丰度比高，而在龈沟液中的丰度比低。然而，通过western印迹仅在防护膜和龈沟液中发现了血清转铁蛋白，其唾液中的丰度比很低。结论我们的研究表明，除了唾液外，龈沟液似乎也有助于形成小薄膜。蛋白质印迹法仅在防护膜和龈沟液中发现唾液，并且唾液中的丰度比很低。结论我们的研究表明，除了唾液外，龈沟液似乎也有助于形成小薄膜。蛋白质印迹法仅在防护膜和龈沟液中发现唾液，并且唾液中的丰度比很低。结论我们的研究表明，除了唾液外，龈沟液似乎也有助于形成小薄膜。