ABSTRACT Osteopontin (OPN) has been shown to play a significant role in regulating the aggressiveness of cancer cells and promote tumor growth. Evaluation of this phosphorylated extracellular glycoprotein expression may help estimate its use as a potential prognostic marker in tumorigenesis of different renal tumors. The objective of the present study was to characterize for the first time the expression pattern of OPN in primary renal tumors and correlate its association to tumor progression and survival. A total of 68 primary renal tumors (clear cell renal cell carcinoma, oncocytoma, renal cell carcinoma, invasive urothelial carcinoma, papillary renal cell carcinoma, chromophobe renal cell carcinoma, papillary urothelial carcinoma) were analyzed by immunohistochemical staining and Western blot methods. Expression of OPN in relation to grading, histologic type of tumor, and survival was statistically assessed. Study data demonstrated that OPN is differentially expressed in various renal tumor cells types. It was shown that OPN is predominantly expressed at the protein level in clear cell renal cell carcinoma when compared to other types of renal tumors. In conclusion, osteopontin may be involved in the pathogenesis of renal tumors. However, the role of OPN expression in predicting the biological response requires further evaluation.

中文翻译：

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骨桥蛋白在肾细胞肿瘤中差异表达

摘要 骨桥蛋白 (OPN) 已被证明在调节癌细胞的侵袭性和促进肿瘤生长方面发挥着重要作用。评估这种磷酸化的细胞外糖蛋白表达可能有助于估计其作为不同肾肿瘤发生的潜在预后标志物的用途。本研究的目的是首次表征 OPN 在原发性肾肿瘤中的表达模式，并将其与肿瘤进展和生存相关联。通过免疫组织化学染色和蛋白质印迹方法分析了总共 68 个原发性肾肿瘤（透明细胞肾细胞癌、嗜酸细胞瘤、肾细胞癌、浸润性尿路上皮癌、乳头状肾细胞癌、嫌色肾细胞癌、乳头状尿路上皮癌）。OPN 与分级相关的表达，肿瘤的组织学类型，并对生存率进行统计评估。研究数据表明，OPN 在各种肾肿瘤细胞类型中差异表达。结果表明，与其他类型的肾肿瘤相比，OPN 在透明细胞肾细胞癌中主要在蛋白质水平上表达。综上所述，骨桥蛋白可能参与了肾肿瘤的发病机制。然而，OPN 表达在预测生物反应中的作用需要进一步评估。骨桥蛋白可能参与肾肿瘤的发病机制。然而，OPN 表达在预测生物反应中的作用需要进一步评估。骨桥蛋白可能参与肾肿瘤的发病机制。然而，OPN 表达在预测生物反应中的作用需要进一步评估。