The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular breeding of oysters. Heat shock transcription factor 1 (HSF1) plays an important role in the thermal stress resistance. However, the regulatory relationship between the expansion of heat shock protein (HSP) HSP 70 and HSF1 is not yet clear in C. gigas. In this study, we analyzed genes regulated by HSF1 in response to heat shock by chromatin immunoprecipitation followed by sequencing (ChIP-seq), determined the expression patterns of target genes by qRT-PCR, and validated the regulatory relationship between one HSP70 and HSF1. We found 916 peaks corresponding to HSF1 binding sites, and these peaks were annotated to the nearest genes. In Gene Ontology analysis, HSF1 target genes were related to signal transduction, energy production, and response to biotic stimulus. Four HSP70 genes, two HSP40 genes, and one small HSP gene exhibited binding to HSF1. One HSP70 with a binding site in the promoter region was validated to be regulated by HSF1 under heat shock. These results provide a basis for future studies aimed at determining the mechanisms underlying thermal tolerance and provide insights into gene regulation in the Pacific oyster.

中文翻译：

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通过ChIP-seq鉴定太平洋牡蛎Crassostrea gigas中涉及热胁迫的HSF1目标基因。

太平洋牡蛎Crassostrea gigas，一种生活在潮间带的重要商业物种，面临巨大的温度波动。因此，重要的是确定候选基因和与耐热性相关的关键调控关系，这可以帮助牡蛎的分子育种。热激转录因子1（HSF1）在抗热应激方面起着重要作用。然而，热休克蛋白的膨胀之间的关系调节（HSP）HSP 70和HSF1尚未在清除C.牡蛎。在这项研究中，我们分析了受HSF1调控的基因通过染色质免疫沉淀再测序（ChIP-seq）响应热休克，通过qRT-PCR确定靶基因的表达模式，并验证一种HSP70和HSF1之间的调节关系。我们发现了916个与HSF1结合位点相对应的峰，这些峰被注释为最接近的基因。在基因本体分析中，HSF1目标基因与信号转导，能量产生以及对生物刺激的反应有关。四个HSP70基因，两个HSP40基因和一个小的HSP基因表现出与HSF1的结合。一台HSP70在启动子区域具有结合位点的蛋白被证实在热激下受HSF1调节。这些结果为将来的研究奠定了基础，这些研究旨在确定潜在的热耐受机制，并为太平洋牡蛎的基因调控提供了见识。