Rapid on-site evaluation (ROSE) is one of cytopathology's "unique selling propositions." The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides.

中文翻译：

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超快甲苯胺蓝染色，用于细胞学涂片的快速现场评估。

快速现场评估（ROSE）是细胞病理学的“独特卖点”之一。所用染色的质量，速度和易于处理是ROSE程序功效的关键因素。在这里，我们描述了快速甲苯胺蓝染色的修改，可以在25 s内完成，提供出色的核形态，并与随后的玻片Papanicolaou染色兼容。此外，由于不需要用于临时安装和盖玻片的酒精基固定剂和甘油以外的有机溶剂，因此可以最大限度地减少与有害化学物质的接触。我们已在ROSE实践中成功使用了该方案，并且未观察到甲苯胺蓝和永久性Papanicolaou染色玻片之间的任何差异。