Effects of meiotic stage and cumulus status on development of equine oocytes after vitrification was evaluated. Immature oocytes with corona radiata (IMM); in vitro-matured oocytes with corona radiata (MAT CR+); and in vitro-matured oocytes denuded of cumulus (MAT CR-) were vitrified using the Cryotech® method. Warming medium was equilibrated either in 5% CO2 or Air. IMM oocytes underwent in vitro maturation after warming. Recovery, survival, and maturation rates, and cleavage and blastocyst rates after ICSI, were evaluated. Recovery was higher for oocytes warmed in CO2- than Air-equilibrated medium (86 ± 3 vs. 76.9 ± 4%, respectively). Maturation for all vitrified-warmed oocyte treatments (37 ± 6.5 to 45.9 ± 5.8%) was not different from control (50 ± 4.1%), except for MAT CR- CO2 (20.3 ± 4.6%). Cleavage for MAT CR- CO2 and Air groups was similar to control (67.7 ± 12.1, 71.4 ± 8.1, and 78 ± 5.3%, respectively). One blastocyst was produced (MAT CR + CO2), representing the first equine blastocyst reported after vitrification of an in vitro-matured oocyte.

中文翻译：

---

未成熟和体外成熟的马卵母细胞玻璃化后的胚胎发育

评估减数分裂阶段和卵丘状态对玻璃化冷冻后马卵母细胞发育的影响。具有放射冠 (IMM) 的未成熟卵母细胞；具有放射冠的体外成熟卵母细胞 (MAT CR+)；使用 Cryotech® 方法玻璃化去除卵丘 (MAT CR-) 的体外成熟卵母细胞。加热介质在 5% CO2 或空气中平衡。IMM 卵母细胞在加热后经历体外成熟。评估 ICSI 后的恢复、存活和成熟率，以及卵裂和囊胚率。在 CO2 中加热的卵母细胞的回收率高于空气平衡培养基（分别为 86 ± 3 和 76.9 ± 4%）。除了 MAT CR-CO2 (20.3 ± 4.6%) 之外，所有玻璃化加热的卵母细胞处理的成熟 (37 ± 6.5 至 45.9 ± 5.8%) 与对照 (50 ± 4.1%) 没有区别。MAT CR-CO2 和 Air 组的裂解与对照相似（分别为 67.7 ± 12.1、71.4 ± 8.1 和 78 ± 5.3%）。产生了一个囊胚（MAT CR + CO2），代表在体外成熟的卵母细胞玻璃化后报道的第一个马囊胚。