Semen extender has a vital role in preservation of sperm cells properties in terms of sperm viability, motility, acrosome integrity, and mitochondrial membrane potential. The objective of the present study was to evaluate a new extender, known as Thai native chicken (TNC) extender compared to BHSV-based and modified Sasaki extenders for freezing chicken semen. Semen from Thai native roosters was collected, pooled, and randomly divided into three groups. Semen was frozen with a simple freezing method using nitrogen vapor and dimethylformamide. In the first experiment, post-thaw motion parameters, viability, acrosome integrity, mitochondrial function, and lipid peroxidation levels were analyzed using computer-assisted sperm analysis, propidium iodide, fluorescein isothiocyanate-conjugate peanut agglutinin, JC-1, and the thiobarbituric acid reaction. Results showed that the type of extender had no effect on the percentage of total motile and curvilinear velocity. The percentage of progressive motile, straight-line velocity, and average path velocity of post-thawed semen were significantly lower in TNC compared to the modified Sasaki extender. However, the percentages of post-thawed acrosome integrity and active mitochondria were significantly higher in TNC extender (P < 0.05). For the second experiment, semen was thawed by using each of extenders thereafter, was inseminated to 48-layer breeder hens to determine the fertility rate. Among the three extenders used, the highest fertility rate was found in TNC extender. In conclusion, TNC extender can be recommended as an appropriate and useful cryopreservation media for native chicken semen since it maintains the quality of rooster semen and fertility after freezing and thawing process.

中文翻译：

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TNC和标准稀释剂对泰国本土鸡精解冻后质量和体内生育力的比较

精液稀释剂在保存精子活力、运动性、顶体完整性和线粒体膜电位方面的精子特性方面起着至关重要的作用。本研究的目的是评估一种新的增量剂，称为泰国本土鸡 (TNC) 增量剂，与基于 BHSV 和改良 Sasaki 冷冻鸡精液的增量剂相比。泰国本土公鸡的精液被收集、汇集并随机分为三组。使用氮气蒸气和二甲基甲酰胺通过简单的冷冻方法冷冻精液。在第一个实验中，使用计算机辅助精子分析、碘化丙啶、异硫氰酸荧光素偶联花生凝集素、JC-1 和硫代巴比妥酸对解冻后运动参数、活力、顶体完整性、线粒体功能和脂质过氧化水平进行了分析反应。结果表明，扩展器的类型对总运动速度和曲线速度的百分比没有影响。与改良 Sasaki 扩展器相比，TNC 中解冻后精液的渐进运动百分比、直线速度和平均路径速度显着降低。然而，TNC 扩展剂中解冻后的顶体完整性和活性线粒体的百分比显着更高（P < 0.05）。对于第二个实验，其后使用每种稀释剂解冻精液，将精液授精给48层种鸡以确定受精率。在使用的三种扩展剂中，TNC 扩展剂的生育率最高。综上所述，