Crude extracts and phytochemical compounds derived from Annona muricata leaves have been demonstrated to exert neuroprotective effects. However, the neuroprotective effects of Annona muricata leaves-derived polysaccharide extracts (ALPs) have not been investigated. ALP treatment was shown to induce concentration-dependent antioxidant activity in HT22 cells, and to increase cell viability in H2O2-treated HT22 cells. These effects were correlated with a decrease in major components of oxidation, including: Ca2+, ROS, and malondialdehyde (MDA). Mediators of the intracellular response to oxidation, including Bax, cytochrome c, and cleaved caspases-3, -8, -9, MAPKs, and NF-κB, were positively influenced by ALP treatment under conditions of H2O2-mediated oxidative stress. In addition, ALP restored the expression of superoxide dismutase (SOD) and associated signaling pathways (PARP, PI3K/AKT and Nrf2-mediated HO-1/NQO-1) following H2O2 treatment. These results provide new pharmacological evidence that ALP facilitates neuroprotection via prevention of neuronal oxidative stress and promotion of cell survival signaling pathways.Abbreviations: ABTS: 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonicacid); AD: Alzheimer's disease; ALP: polysaccharide extracts isolated from Annona muricata leaves; ARE: antioxidant response element; DPPH: 1,1-diphenyl-picrylhydrazyl; DCFH-DA: 2',7'-dichlorofluorescin diacetate; ECL: electrochemiluminescence; ERK: extracellular regulated kinase; FBS: Fetal bovine serum; FITC: fluorescein isothiocyanate; FRAP: ferric reducing antioxidant power; HO-1: Heme oxygenase-1; JNK: c-jun N-terminal kinase; MAPKs: mitogen-activated protein kinases; MDA: malondialdehyde; MMP: mitochondrial membrane potential; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide; NQO1: NAD(P)H:quinine oxidoreductase 1, Nrf2: nuclear factor-E2-related factor 2; PD: parkinson's disease; PI3K: phosphatidylinositol-3kinase; PVDF: polyvinylidene difluoride; ROS: reactive oxygen species; SOD: Superoxidedismutase; TPTZ: tripydyltriazine.

中文翻译：

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Annona muricata衍生的多糖对过氧化氢诱导的神经元HT22细胞损伤的神经保护作用。

已经证明，从栗色Annona muricata叶片中提取的粗提物和植物化学化合物具有神经保护作用。但是，尚未研究过番荔枝番荔枝叶衍生的多糖提取物（ALP）的神经保护作用。已显示ALP处理可诱导HT22细胞中浓度依赖性的抗氧化活性，并增加H2O2处理的HT22细胞中的细胞活力。这些影响与氧化的主要成分减少有关，这些主要成分包括：Ca2 +，ROS和丙二醛（MDA）。在H2O2介导的氧化应激条件下，ALP处理对包括Bax，细胞色素c和裂解的caspases-3，-8，-9，MAPK和NF-κB在内的细胞内氧化反应的介导因子产生了积极影响。此外，H2O2处理后，ALP恢复了超氧化物歧化酶（SOD）和相关信号通路（PARP，PI3K / AKT和Nrf2介导的HO-1 / NQO-1）的表达。这些结果提供了新的药理学证据，表明ALP通过预防神经元氧化应激和促进细胞存活信号通路促进神经保护作用。AD：阿尔茨海默氏病；ALP：从番荔枝番荔枝叶中分离的多糖提取物；分别是：抗氧化反应元素；DPPH：1，1-二苯基-吡啶并肼基；DCFH-DA：2'，7'-二氯荧光素二乙酸盐；ECL：电化学发光；ERK：细胞外调节激酶；FBS：胎牛血清；FITC：异硫氰酸荧光素；FRAP：铁还原抗氧化能力；HO-1：血红素加氧酶-1；JNK：c-jun N-末端激酶；MAPK：有丝分裂原激活的蛋白激酶；MDA：丙二醛；MMP：线粒体膜电位；MTT：3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物；NQO1：NAD（P）H：奎宁氧化还原酶1，Nrf2：核因子-E2相关因子2；PD：帕金森氏病；PI3K：磷脂酰肌醇3激酶；PVDF：聚偏二氟乙烯；ROS：活性氧；SOD：超氧化物歧化酶；TPTZ：三苯甲基三嗪。超氧化物歧化酶; TPTZ：三苯甲基三嗪。超氧化物歧化酶; TPTZ：三苯甲基三嗪。