Pituitary adenomas constitute one of the most common intracranial tumors. MicroRNAs play an important role in development and progression of pituitary adenomas. In this study, we showed that miR-219a-2-3p was significantly down-regulated in pituitary adenomas cells. Overexpression of miR-219a-2-3p suppressed the proliferation and promoted apoptosis of pituitary adenomas cells. After bioinformatics analysis, we found that MDM2 was one of the downstream targets of miR-219a-2-3p. Further researches showed that miR-219a-2-3p could reduce the protein level of MDM2 by binding to the 3'-UTR of MDM2 and promoted p53 expression. Then, we overexpressed both miR-219a-2-3p and MDM2 in the same group and found that it could counteract the effect of overexpressing miR-219a-2-3p alone on proliferation and apoptosis of pituitary adenoma cells. Taken together, these results suggested that miR-219a-2-3p regulated the proliferation and apoptosis by targeting MDM2/p53 in pituitary adenomas. Therefore, miR-219a-2-3p may serve as a novel marker and therapeutic target for pituitary adenomas.

中文翻译：

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MiR-219a-2-3p通过靶向垂体腺瘤细胞中的MDM2 / p53抑制细胞增殖并促进凋亡。

垂体腺瘤是最常见的颅内肿瘤之一。MicroRNA在垂体腺瘤的发生和发展中起重要作用。在这项研究中，我们表明miR-219a-2-3p在垂体腺瘤细胞中显着下调。miR-219a-2-3p的过表达抑制垂体腺瘤细胞的增殖并促进其凋亡。经过生物信息学分析，我们发现MDM2是miR-219a-2-3p的下游靶标之一。进一步的研究表明，miR-219a-2-3p可以通过与MDM2的3'-UTR结合并促进p53表达来降低MDM2的蛋白水平。然后，我们在同一组中过表达miR-219a-2-3p和MDM2，发现它可以抵消过表达miR-219a-2-3p对垂体腺瘤细胞增殖和凋亡的影响。在一起 这些结果表明，miR-219a-2-3p通过靶向垂体腺瘤中的MDM2 / p53来调节增殖和凋亡。因此，miR-219a-2-3p可以作为垂体腺瘤的新型标志物和治疗靶标。