Abstract

This study was performed with 40 (20 males, 20 females) BALB/c mice divided into 4 experimental groups and a control group, each consisting of 8 mice (4 males, 4 females). Experimental groups were administered 470, 620, 940, and 1880 mg/kg doses of steviol glycosides, orally, for 4 weeks. The total antioxidants and the oxidant status, paraoxonase-1 enzyme activity, high density lipoprotein-cholesterol, and low-density lipoprotein-cholesterol levels were analyzed from blood samples. The chromosomal aberrations and cell cycle activities were examined from bone marrow samples. Plasma lipid parameters were not affected by the dose of steviol glycosides, however, the total antioxidants, oxidant status, and paraoxonase-1 enzyme activity were found to be negatively correlated with the doses. A positive correlation was found between the total oxidant status and the dose (r = 0.65) and between the mitotic index and the dose (r = 0.74). The dose of steviol glycosides also increased the percentage of the abnormal cells and the CA/cell dose in a dependent manner (r = 0.74 and 0.76, respectively). The study findings concluded that steviol glycosides slightly increased the oxidative damage, cell cycle activity, and chromosomal aberration frequency. However, we did not evaluate the potential of steviol glycosides as genotoxic and mitogenic agents, and, therefore, further investigations are required.

CAS number: 58543–16-1

摘要

本研究采用 40 只（20 只雄性，20 只雌性）BALB/c 小鼠进行，分为 4 个实验组和一个对照组，每组由 8 只小鼠（4 只雄性，4 只雌性）组成。实验组口服 470、620、940 和 1880 mg/kg 剂量的甜菊醇糖苷，持续 4 周。从血液样本中分析总抗氧化剂和氧化状态、对氧磷酶-1 酶活性、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇水平。从骨髓样本中检查染色体畸变和细胞周期活性。血浆脂质参数不受甜菊醇糖苷剂量的影响，但发现总抗氧化剂、氧化状态和对氧磷酶-1 酶活性与剂量呈负相关。r  = 0.65）和有丝分裂指数和剂量之间（r  = 0.74）。甜菊醇糖苷的剂量也以依赖的方式增加了异常细胞的百分比和 CA/细胞剂量（分别为r  = 0.74 和 0.76）。研究结果得出结论，甜菊醇糖苷略微增加了氧化损伤、细胞周期活性和染色体畸变频率。然而，我们没有评估甜菊糖苷作为遗传毒性和促有丝分裂剂的潜力，因此需要进一步研究。

CAS 号： 58543–16-1