Several investigations have recently assessed the ability of some aquatic invertebrates to act as tools for avian influenza A virus (IAV) surveillance as well as their potential role(s) in IAV ecology. Because of this, as well as the high IAV seroprevalence rates noted in select mesocarnivores that commonly inhabit aquatic and semi-aquatic habitats, we evaluated the effects that freshwater crayfish have on IAV in water at three dose levels and monitored for the presence of IAV in crayfish tissues (gill and green gland) and haemolymph at multiple time points. At relatively high, medium and low (approximately 104 , 103 and 102 EID50 /ml, respectively) doses, mesocosms containing crayfish (Orconectes sp.) had less detectable IAV RNA present when final water samples were assayed (9 days post-contact [DPC]). In general, containers without crayfish present had nearly three-fold greater quantities of viral RNA at 9 DPC. A varying number of RNA positive samples were detected for the three crayfish sample types collected. Gill tissue produced the largest number of positive non-water samples (n = 26), with the highest quantities detected from crayfish sampled on 1 and 4 DPC (103.5 EID50 equivalent/ml). On a few occasions, gill (n = 8) and haemolymph samples (n = 1) produced higher quantities of viral RNA than their respective water samples or water samples collected 1-2 DPC earlier, but these differences were typically minor. Based upon water samples, statistical models indicated that the interaction of dose and crayfish exposure days explained most of the variation in these data. Future efforts should address if crayfish exposed to IAV-laden water have the capacity to successfully transmit IAVs to mammals and birds which frequently prey upon them.

中文翻译：

---

淡水小龙虾对水中甲型流感病毒持续性的影响。

最近的一些研究评估了一些水生无脊椎动物充当禽流感A病毒（IAV）监视工具的能力及其在IAV生态学中的潜在作用。因此，以及在通常栖息于水生和半水生生境的某些中生食肉动物中发现的高IAV血清阳性率，我们评估了三种剂量水平下淡水小龙虾对水中IAV的影响并监测了IAV在水中的存在。小龙虾组织（g和绿腺）和血淋巴在多个时间点。在相对高，中和低（分别为104、103和102 EID50 / ml）剂量下，当对最终水样进行分析时（接触后9天[DPC]，含有小龙虾（Orconectes sp。）的中型小肠中IAV RNA的含量较低。 ]）。一般来说，不存在小龙虾的容器在9 DPC处的病毒RNA量几乎增加了三倍。对于收集的三种小龙虾样品类型，检测到不同数量的RNA阳性样品。组织产生的阳性非水样品数量最多（n = 26），在1和4个DPC上从小龙虾中检出的数量最高（103.5 EID50当量/ ml）。在少数情况下，g（n = 8）和血淋巴样品（n = 1）产生的病毒RNA数量要比各自的水样品或早于1-2 DPC的水样品高，但这些差异通常很小。基于水样本，统计模型表明剂量和小龙虾接触天数的相互作用解释了这些数据的大部分变化。