Based on the reversible covalent binding between borates and glycoproteins, we proposed a scheme that can specifically recognize and detect immunoglobulin G (IgG). The application of magnetic materials to enrich SERS probes can provide an enhanced signal and repeatability. The results showed that the prepared sandwich-like complex displayed high sensitivity and excellent selectivity for IgG. The Raman peak intensity showed a good linear relationship in the concentration range of 1 mg/mL – 1 ng/mL. The linear equation was y = 657.93x + 2963.9, R² = 0.996. Parallel testing of this sandwich-like complex proved to have excellent repeatability. This method provided a new possibility for clinical non-immunity and the quantitative detection of IgG.

基于硼酸盐与糖蛋白之间的可逆共价结合，我们提出了一种可以特异性识别和检测免疫球蛋白G（IgG）的方案。应用磁性材料丰富 SERS 探针可以提供增强的信号和可重复性。结果表明，所制备的夹心复合物对IgG表现出高灵敏度和优异的选择性。拉曼峰强度在1 mg/mL – 1 ng/mL的浓度范围内表现出良好的线性关系。线性方程为y = 657.93 x + 2963.9，R ² = 0.996。这种三明治状复合物的并行测试证明具有出色的重复性。该方法为临床非免疫及IgG的定量检测提供了新的可能。