Boehmeria tricuspis (Hance) Makino constitutes a hardy herbaceous or shrubby perennial native to East Asia that includes different ploidy levels and reproductive modes (diplosporous to sexual). Although several apomeiosis-associated genes have been described, the genetic control and molecular mechanisms underlying apomeiosis remain poorly understood. Moreover, the basis of the correlation between polyploidy and apomixis has not yet been clarified. We utilized long-read sequencing to produce a full-length reference floral transcriptome of B. tricuspis. Based on the generated database, gene expression of the female flowers of different ploidy levels and reproductive mode cytotypes was compared. Overall, 1,387 genes related to apomeiosis, 217 genes related to ploidy, and 9 genes associated with both apomixis and ploidy were identified. Gene Ontology analyses of this set of transcripts indicated reproductive genes, especially those related to “cell differentiation” and “cell cycle process,” as significant factors regulating apomeiosis. Furthermore, our results suggested that different expressions of stress response genes might be important in the preparation for apomeiosis transition. In addition, our observations indicated that the expression of apomeiosis may not depend on polyploidy but rather on deregulation of the sexual pathway in B. tricuspis.

中文翻译：

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苎麻的全长参考花转录组提供了对无性减数分裂和多倍体的见解。


Boehmeria tricuspis (Hance) Makino 是一种原产于东亚的耐寒草本或灌木多年生植物，包括不同的倍性水平和生殖模式（双孢子到有性）。尽管已经描述了一些与减数分裂相关的基因，但减数分裂背后的遗传控制和分子机制仍然知之甚少。此外，多倍体与无融合生殖之间相关性的基础尚未阐明。我们利用长读长测序来生成B的全长参考花转录组。三尖瓣。基于生成的数据库，比较了不同倍性水平和生殖模式细胞型的雌花的基因表达。总体而言，鉴定了 1,387 个与无性生殖相关的基因、217 个与倍性相关的基因以及 9 个与无融合生殖和倍性相关的基因。对这组转录本的基因本体分析表明，生殖基因，特别是那些与“细胞分化”和“细胞周期过程”相关的基因，是调节无减数分裂的重要因素。此外，我们的结果表明，应激反应基因的不同表达可能在无减数分裂过渡的准备中很重要。此外，我们的观察表明，无减数分裂的表达可能不依赖于多倍体，而是依赖于性途径的失调。三尖瓣。