Boehmeria tricuspis (Hance) Makino constitutes a hardy herbaceous or shrubby perennial native to East Asia that includes different ploidy levels and reproductive modes (diplosporous to sexual). Although several apomeiosis-associated genes have been described, the genetic control and molecular mechanisms underlying apomeiosis remain poorly understood. Moreover, the basis of the correlation between polyploidy and apomixis has not yet been clarified. We utilized long-read sequencing to produce a full-length reference floral transcriptome of B. tricuspis. Based on the generated database, gene expression of the female flowers of different ploidy levels and reproductive mode cytotypes was compared. Overall, 1,387 genes related to apomeiosis, 217 genes related to ploidy, and 9 genes associated with both apomixis and ploidy were identified. Gene Ontology analyses of this set of transcripts indicated reproductive genes, especially those related to “cell differentiation” and “cell cycle process,” as significant factors regulating apomeiosis. Furthermore, our results suggested that different expressions of stress response genes might be important in the preparation for apomeiosis transition. In addition, our observations indicated that the expression of apomeiosis may not depend on polyploidy but rather on deregulation of the sexual pathway in B. tricuspis.

中文翻译：

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中华三叶草的全长参考花卉转录组提供了对无融合生殖和多倍体的见解。

Boehmeria tricuspis（Hance）Makino是东亚地区多年生的强壮草本或灌木状植物，具有不同的倍性水平和生殖方式（双性到有性）。尽管已经描述了几种与减数分裂相关的基因，但对减数分裂的遗传控制和分子机制仍然知之甚少。此外，多倍体与无融合生殖之间相关性的基础尚未阐明。我们利用长时间阅读测序来产生B的全长参考花卉转录组。cus。基于生成的数据库，比较了不同倍性水平和生殖模式细胞类型的雌花的基因表达。总体上，鉴定出与无融合生殖相关的1,387个基因，与倍性相关的217个基因以及与无融合生殖和倍性相关的9个基因。这组转录本的基因本体分析表明，生殖基因，特别是与“细胞分化”和“细胞周期过程”有关的基因，是调节细胞凋亡的重要因素。此外，我们的研究结果表明应激反应基因的不同表达可能在无融合生殖过渡的准备中很重要。此外，我们的观察表明，Apomeiosis的表达可能不依赖于多倍体，而取决于B中性途径的失调。cus。