BACKGROUND Cerebrospinal fluid (CSF) analysis aids in categorizing underlying disease processes in patients with neurologic disease. Convention suggests that CSF should be collected caudal to the lesion. However, little evidence exists to justify this assertion. HYPOTHESIS/OBJECTIVES Evaluate the clinicopathologic differences between CSF collected from the cerebellomedullary (CM) and lumbar cisterns in dogs presented for evaluation of neurologic disease. ANIMALS Fifty-one client-owned dogs undergoing magnetic resonance imaging (MRI) and CSF collection for investigation of neurologic disease. METHODS Cerebrospinal fluid was prospectively collected from the CM and lumbar cisterns in all patients. The total protein (TP) concentration, red blood cell (RBC) count, and total nucleated cell count (TNCC) were analyzed within 30 minutes of collection. Results and cytology findings were interpreted by a single pathologist. RESULTS Fifty-one paired samples were collected. The TNCC (P < .001), RBC (P < .001), and TP (P < .001) were different between collection sites. When grouped by neurolocalization, TP (intracranial, P < .001; cervical, P < .001; thoracolumbar, P < .001) and RBC (intracranial, P < .001; cervical, P ≤ .002; thoracolumbar, P = .006) counts were significantly different. The TNCC was significantly different in the cervical (P = .04) and thoracolumbar localizations (P = .004) but not for intracranial (P = .30) localizations. The pathologist's interpretation differed between sites in 66.7% of the cases (34/51). CONCLUSIONS In dogs with lesions that neurolocalized to the brain or cervical spinal cord, there may be clinical benefit in collecting fluid from both the CM and lumbar cisterns. In dogs with thoracolumbar myelopathy, CSF collected from the CM cistern may not be representative of the underlying disease process.

中文翻译：

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患有神经系统疾病的犬脑脊髓和腰椎脑脊液分析的比较。

背景技术脑脊液（CSF）分析有助于对神经系统疾病患者的潜在疾病过程进行分类。常规建议应在病灶尾部收集脑脊液。但是，几乎没有证据可以证明这一主张。假设/目标评价从评估为神经系统疾病评估的犬小脑髓（CM）和腰部储水池收集的CSF的临床病理差异。动物51只客户拥有的狗正在接受磁共振成像（MRI）和CSF收集，以调查神经系统疾病。方法前瞻性收集所有患者的CM和腰部池。在收集的30分钟内分析了总蛋白质（TP）浓度，红细胞（RBC）计数和总有核细胞计数（TNCC）。结果和细胞学检查结果由一名病理学家解释。结果收集了51对样本。收集地点之间的TNCC（P <.001），RBC（P <.001）和TP（P <.001）不同。按神经定位分为TP（颅内，P <.001;颈椎，P <.001;胸腰椎，P <.001）和RBC（颅内，P <.001;颈椎，P≤.002;胸腰椎，P =。 006）计数显着不同。TNCC在子宫颈（P = .04）和胸腰椎定位（P = .004）方面有显着差异，但对于颅内（P = .30）定位则没有显着差异。在66.7％的病例中，病理学家的解释在部位之间有所不同（34/51）。结论在具有神经定位于脑或颈脊髓的病变的狗中，从CM和腰部水箱中收集液体可能会有临床益处。在患有胸腰椎脊髓病的狗中，从CM水箱收集的CSF可能不代表潜在的疾病过程。