In the current study, we sought to investigate whether T-type Ca²⁺ channels (TCCs) in the brain are involved in generating post-anesthetic hyperexcitatory behaviors (PAHBs). We found that younger rat pups (postnatal days 9–11) had a higher incidence of PAHBs and higher PAHB scores than older pups (postnatal days 16–18) during emergence from sevoflurane anesthesia. The power spectrum of the theta oscillations (4 Hz–8 Hz) in the prefrontal cortex was significantly enhanced in younger pups when PAHBs occurred, while there were no significant changes in older pups. Both the power of theta oscillations and the level of PAHBs were significantly reduced by the administration of TCC inhibitors. Moreover, the sensitivity of TCCs in the medial dorsal thalamic nucleus to sevoflurane was found to increase with age by investigating the kinetic properties of TCCs in vitro. TCCs were activated by potentiated GABAergic depolarization with a sub-anesthetic dose of sevoflurane (1%). These data suggest that (1) TCCs in the brain contribute to the generation of PAHBs and the concomitant electroencephalographic changes; (2) the stronger inhibitory effect of sevoflurane contributes to the lack of PAHBs in older rats; and (3) the contribution of TCCs to PAHBs is not mediated by a direct effect of sevoflurane on TCCs.

中文翻译：

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T型钙通道在新生大鼠七氟醚麻醉过程中产生超兴奋行为中的作用。

在本研究中，我们试图调查T型Ca ²⁺是否大脑中的通道（TCC）参与麻醉后过度兴奋行为（PAHBs）的产生。我们发现，七氟醚麻醉期间，幼小幼崽（出生后9-11天）的PAHBs发生率较高，PAHB评分也更高（大生幼崽16-18天）。当幼年幼崽发生PAHBs时，前额叶皮层theta振荡的功率谱（4 Hz–8 Hz）显着增强，而幼龄幼崽则无明显变化。通过使用TCC抑制剂，θ振荡的功率和PAHBs的水平均显着降低。此外，通过研究体外TCCs的动力学特性，发现内侧丘脑内侧核中TCCs对七氟醚的敏感性会随着年龄的增长而增加。。TCCs通过亚麻醉剂量的七氟醚（1％）增强的GABA能去极化激活。这些数据表明：（1）大脑中的TCC促进了PAHBs的产生和随之而来的脑电图变化；（2）七氟醚的较强抑制作用导致老年大鼠缺乏PAHBs；（3）TCC对PAHBs的贡献不是由七氟醚对TCC的直接作用介导的。