Acetyl-hexapeptide 3 (AHP-3) is a small peptide with good anti-wrinkle efficacy and safety profile. However, due to its hydrophilicity and high molecular weight, its skin permeation is generally poor. Innovative microneedle (MN) patch such as the curved, flexible or personalised MN patch is a viable avenue to deliver AHP-3. However, the well-researched geometrical relationship of MN on flat MN patch cannot be assumed for these novel MN patches due to a complex mix of axial and shear forces. In this study, 3D printing was used for the fabrication of various MN patch with different MN geometries and curvatures. Both mechanical strength and skin penetration efficiency were used to determine the optimal MN geometry. The optimal MN geometry was then applied into the fabrication of a personalized MN patch (PMNP) for anti-wrinkle therapy, via 3D printing. In all, the general principles of MN geometrical effects on mechanical strength and skin penetration efficiency for curved and flat MN patch were similar. A MN height of 800 μm, tip diameter of 100 μm, interspacing of 800 μm and base diameter of 400 μm was observed to be the optimal MN geometry across all curvatures. In vitro skin permeation study demonstrated enhanced transdermal delivery of AHP-3 using the fabricated PMNP. Therefore, PMNP with optimized MN geometry can potentially be a novel approach to augment transdermal delivery of AHP-3 for effective wrinkle management.

中文翻译：

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个性化微针眼贴片的几何优化，用于抗皱小肽的透皮递送。

乙酰六肽3（AHP-3）是一种小肽，具有良好的抗皱功效和安全性。但是，由于其亲水性和高分子量，其皮肤渗透性通常较差。创新的微针（MN）贴片（例如弯曲的，灵活的或个性化的MN贴片）是提供AHP-3的可行途径。然而，由于轴向力和剪切力的复杂混合，对于这些新颖的MN斑块，不能假设MN在平坦MN斑块上的研究良好的几何关系。在这项研究中，将3D打印用于制造具有不同MN几何形状和曲率的各种MN贴片。机械强度和皮肤渗透效率均用于确定最佳MN几何形状。然后，通过3D打印将最佳的MN几何形状应用于个性化MN贴片（PMNP）的制造中，以进行抗皱治疗。总之，MN几何效应对弯曲和平坦MN贴片的机械强度和皮肤渗透效率的一般原理是相似的。观察到在所有曲率上，MN高度为800μm，尖端直径为100μm，间距为800μm，基部直径为400μm是最佳的MN几何形状。体外皮肤渗透研究表明，使用制造的PMNP可以增强AHP-3的透皮递送。因此，具有优化的MN几何形状的PMNP可能是增加AHP-3透皮递送以有效控制皱纹的新颖方法。观察到800μm的间距和400μm的基本直径是所有曲率的最佳MN几何形状。体外皮肤渗透研究表明，使用制造的PMNP可以增强AHP-3的透皮递送。因此，具有优化的MN几何形状的PMNP可能是增加AHP-3透皮递送以有效控制皱纹的新方法。观察到800μm的间距和400μm的基本直径是所有曲率的最佳MN几何形状。体外皮肤渗透研究表明，使用制造的PMNP可以增强AHP-3的透皮递送。因此，具有优化的MN几何形状的PMNP可能是增加AHP-3透皮递送以有效控制皱纹的新方法。