As a member of parabens (PBs), Isobutylparaben (IBP) has a broad-spectrum antimicrobial activity and widely used in personal care products and cosmetics. Recent studies have indicated that usage of IBP poses a potential threat to reproductive health. In this study, we aimed to reveal the effects of acute exposure to IBP on the meiotic maturation of porcine cumulus oocyte complexes. Initial study showed that 200 μM of IBP significantly reduced the rate of the first polar body extrusion with no significant effect on cumulus cell expansion; however, 400 μM of IBP could significantly affect both. Further research revealed that abnormal spindles, misalignment chromosomes, and aberrant distributed actin filaments were detected in IBP-treated oocytes, which indicates that the cytoskeleton architecture of oocyte could be the target of IBP. At the same time, ROS level and apoptosis rate of oocyte were significantly increased by IBP exposure. Moreover, the levels of H3K9me3 and H3K27me3 were significantly induced in oocytes by IBP. Collectively, these results demonstrate that acute exposure to IBP could disrupt porcine oocyte maturation through affecting cytoskeleton, oxidative stress, viability and epigenetic modification. Environ. Mol. Mutagen. 2020. © 2020 Wiley Periodicals, Inc.

中文翻译：

---

对羟基苯甲酸异丁酯通过增加氧化应激和细胞骨架异常而负面影响猪卵母细胞成熟。

作为对羟基苯甲酸酯（PBs）的成员，对羟基苯甲酸异丁酯（IBP）具有广谱抗菌活性，并广泛用于个人护理产品和化妆品中。最近的研究表明，使用IBP对生殖健康构成潜在威胁。在这项研究中，我们旨在揭示急性暴露于IBP对猪卵丘卵母细胞复合物减数分裂成熟的影响。初步研究表明，200μM的IBP显着降低了第一极体挤出的速率，而对卵丘细胞的膨胀没有显着影响。但是，400μM的IBP可能会同时影响两者。进一步的研究表明，在IBP处理的卵母细胞中检测到异常的纺锤体，染色体错位和异常分布的肌动蛋白丝，这表明卵母细胞的细胞骨架结构可能是IBP的靶标。同时，IBP暴露显着增加了ROS水平和卵母细胞凋亡率。此外，IBP在卵母细胞中显着诱导了H3K9me3和H3K27me3的水平。总的来说，这些结果表明，急性暴露于IBP可以通过影响细胞骨架，氧化应激，生存力和表观遗传修饰来破坏猪卵母细胞的成熟。环境。大声笑 诱变剂。2020。©2020 Wiley Periodicals，Inc.。