The primary goal of bioprocess cell line development is to obtain high product yields from robustly growing and well-defined clonal cell lines in timelines measured in weeks rather than months. Likewise, high-throughput screening of B cells and hybridomas is required for most cell line engineering workflows. A substantial bottleneck in these processes is detecting and isolating rare clonal cells with the required characteristics. Traditionally, this was achieved by the resource-intensive method of limiting dilution cloning, and more recently aided by semiautomated technologies such as cell sorting (e.g., fluorescence-activated cell sorting) and colony picking. In this paper we report on our novel Cyto-Mine Single Cell Analysis and Monoclonality Assurance System, which overcomes the limitations of current technologies by screening hundreds of thousands of individual cells for secreted target proteins, and then isolating and dispensing the highest producers into microtiter plate wells (MTP). The Cyto-Mine system performs this workflow using a fully integrated, microfluidic Cyto-Cartridge. Critically, all reagents and Cyto-Cartridges used are animal component-free (ACF) and sterile, thus allowing fast, robust, and safe isolation of desired cells.

中文翻译：

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Cyto-Mine：用于高通量单细胞分析、分选、分配和单克隆性保证的集成微滴系统。

生物过程细胞系开发的主要目标是在以数周而不是数月为单位测量的时间线内，从生长旺盛且定义明确的克隆细胞系中获得高产量。同样，大多数细胞系工程工作流程都需要对 B 细胞和杂交瘤进行高通量筛选。这些过程中的一个重大瓶颈是检测和分离具有所需特征的稀有克隆细胞。传统上，这是通过有限稀释克隆的资源密集型方法实现的，最近通过细胞分选（例如，荧光激活细胞分选）和集落挑选等半自动化技术辅助。在本文中，我们报告了我们新颖的 Cyto-Mine 单细胞分析和单克隆性保证系统，它通过筛选数十万个单个细胞的分泌靶蛋白，然后分离最高生产者并将其分配到微量滴定板孔 (MTP) 中来克服当前技术的局限性。Cyto-Mine 系统使用完全集成的微流体 Cyto-Cartridge 执行此工作流程。至关重要的是，所用的所有试剂和细胞滤筒均不含动物成分 (ACF) 且无菌，因此可以快速、稳健且安全地分离所需细胞。