The genome of Acidiphilium multivorum strain AIU 301, acidophilic, aerobic Gram-negative bacteria, was investigated for potential metabolic pathways associated with organic acid production and metal uptake. The genome was compared to other acidic mine drainage isolates, Acidiphilium cryptum JF-5 and Acidithiobacillus ferrooxidans ATCC 23270, as well as Acetobacter pasteurianus 386B, which ferments cocoa beans. Plasmids between two Acidiphilium spp. were compared, and only two of the sixteen plasmids were identified as potentially similar. Comparisons of the genome size to the number of protein coding sequences indicated that A. multivorum and A. cryptum follow the line of best fit unlike A. pasteurianus 386B, which suggests that it was improperly annotated in the database. Pathways between these four species were analyzed bioinformatically and are discussed here. A. multivorum AIU 301, shares pathways with A. pasteurianus 386B including aldehyde and alcohol dehydrogenase pathways, which are used in the generation of vinegar. Mercury reductase, arsenate reductase and sulfur utilization proteins were identified and discussed at length. The absence of sulfur utilization proteins from A. multivorum AIU 301 suggests that this species uses previously undefined pathways for sulfur acquisition. Bioinformatic examination revealed novel pathways that may benefit commercial fields including acetic acid production and biomining.

中文翻译：

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对酸性矿山排水物种Acidiphilium multivorum进行计算机基因组分析，用于潜在的商业乙酸生产和生物开采。

研究了嗜酸，需氧的革兰氏阴性细菌多嗜酸性嗜酸杆菌菌株AIU 301的基因组，寻找与有机酸产生和金属吸收有关的潜在代谢途径。将基因组与其他酸性矿山排水分离株，酸隐孢子虫JF-5和铁氧化酸硫硫杆菌ATCC 23270以及发酵可可豆的巴斯德醋杆菌386B进行了比较。两个嗜酸菌属之间的质粒。比较了16个质粒中只有两个被鉴定为潜在相似。基因组大小与蛋白质编码序列数量的比较表明，与A. pasturianus 386B不同，multivorum和A. cryptum遵循最佳拟合线，这表明数据库中的注释不正确。对这四个物种之间的途径进行了生物信息学分析，并在此进行讨论。A. multivorum AIU 301与A. pasturianus 386B共有通路，包括醛和醇脱氢酶通路，这些通路用于醋的生产。汞还原酶，砷酸还原酶和硫利用蛋白已得到鉴定和详细讨论。A. multivorum AIU 301中不存在硫利用蛋白，这表明该物种使用以前不确定的途径进行硫的获取。生物信息学检查揭示了可能有益于商业领域的新途径，包括乙酸生产和生物开采。鉴定并详细讨论了砷酸还原酶和硫利用蛋白。A. multivorum AIU 301中不存在硫利用蛋白，这表明该物种使用以前不确定的途径进行硫的获取。生物信息学检查揭示了可能有益于商业领域的新途径，包括乙酸生产和生物开采。鉴定并详细讨论了砷酸还原酶和硫利用蛋白。A. multivorum AIU 301中不存在硫利用蛋白，这表明该物种使用以前不确定的途径进行硫的获取。生物信息学检查揭示了可能有益于商业领域的新途径，包括乙酸生产和生物开采。