Abstract

The aim of this study was to examine pancreatic lesions and the possible prophylactic effects of agomelatine (AGO) in lipopolysaccharide (LPS)-induced sepsis in rats. Twenty-four female, 1-year-old Wistar albino rats were divided into three groups: group I (control), group II (study group; 5 mg/kg LPS i.p., single dose), and group III (treatment group; LPS + AGO, single dose p.o., 20 mg/kg AGO + 5 mg/kg LPS, 30 minutes after AGO treatment). The rats were sacrificed six hours after LPS administration. At the necropsy, blood and pancreatic tissue samples were collected for biochemical, pathological, and immunohistochemical analyses. The results showed that LPS caused an increase in serum amylase and lipase levels and a decrease in glucose levels. Histopathological analysis revealed infiltration of numerous neutrophils in pancreatic interstitial tissue and in vessels. In addition, slight vacuoles indicating degenerative changes were observed in endocrine and exocrine pancreatic tissues. Increased caspase-8, haptoglobin (Hp), IL-4, and IL-10 and decreased SIRT-1 expression was observed in both endocrine and exocrine parts of the pancreas in the LPS group. AGO ameliorated the biochemical, histopathological, and immunohistochemical findings. The present study results revealed that LPS-induced pancreatic damage to both endocrine and exocrine cells. In contrast, AGO had ameliorative effects on both biochemical and pathological findings in rats.

摘要

本研究的目的是检查胰腺病变和阿戈美拉汀 (AGO) 在脂多糖 (LPS) 诱导的大鼠脓毒症中可能的预防作用。将 24 只 1 岁雌性 Wistar 白化大鼠分为三组：I 组（对照组）、II 组（研究组；5 mg/kg LPS ip，单剂量）和 III 组（治疗组；LPS + AGO，单剂量口服，20 mg/kg AGO + 5 mg/kg LPS，AGO 治疗后 30 分钟）。LPS给药后6小时处死大鼠。在尸检时，收集血液和胰腺组织样本用于生化、病理学和免疫组织化学分析。结果表明，LPS 导致血清淀粉酶和脂肪酶水平升高，葡萄糖水平降低。组织病理学分析显示胰腺间质组织和血管中大量中性粒细胞浸润。此外，在内分泌和外分泌胰腺组织中观察到表明退行性变化的轻微空泡。在 LPS 组的胰腺内分泌和外分泌部分均观察到 caspase-8、触珠蛋白 (Hp)、IL-4 和 IL-10 增加，SIRT-1 表达降低。AGO 改善了生化、组织病理学和免疫组化结果。本研究结果表明，LPS 诱导的胰腺内分泌和外分泌细胞均受到损伤。相反，AGO 对大鼠的生化和病理结果均有改善作用。在 LPS 组的胰腺内分泌和外分泌部分均观察到 caspase-8、触珠蛋白 (Hp)、IL-4 和 IL-10 增加，SIRT-1 表达降低。AGO 改善了生化、组织病理学和免疫组化结果。本研究结果表明，LPS 诱导的胰腺内分泌和外分泌细胞均受到损伤。相反，AGO 对大鼠的生化和病理结果均有改善作用。在 LPS 组的胰腺内分泌和外分泌部分均观察到 caspase-8、触珠蛋白 (Hp)、IL-4 和 IL-10 增加，SIRT-1 表达降低。AGO 改善了生化、组织病理学和免疫组化结果。本研究结果表明，LPS 诱导的胰腺内分泌和外分泌细胞均受到损伤。相反，AGO 对大鼠的生化和病理结果均有改善作用。