A highly sensitive, non-invasive, and rapid HBV (Hepatitis B virus) screening method combining membrane protein purification with silver nanoparticle-based surface-enhanced Raman scattering (SERS) spectroscopy was developed in this study. Reproducible serum protein SERS spectra were obtained from cellulose acetate membrane-purified human serum from 94 HBV patients and 89 normal groups. Tentative assignments of serum protein SERS spectra showed that the HBV patients primarily led to specific biomedical changes of serum protein. Principal components analysis and linear discriminate analysis were introduced to analyse the obtained spectra, with the diagnostic sensitivity of 92.6% and specificity of 77.5% were achieved for differentiating HBV patients from normal groups.

中文翻译：

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醋酸纤维素膜纯化与表面增强拉曼光谱相结合的血清分析方法用于非侵入性HBV筛查。

本研究开发了一种将膜蛋白纯化与基于银纳米颗粒的表面增强拉曼散射 (SERS) 光谱相结合的高灵敏度、非侵入性和快速 HBV（乙型肝炎病毒）筛查方法。从 94 名 HBV 患者和 89 个正常组的醋酸纤维素膜纯化的人血清中获得可重复的血清蛋白 SERS 光谱。血清蛋白 SERS 光谱的初步分配表明，HBV 患者主要导致血清蛋白的特定生物医学变化。采用主成分分析和线性判别分析对得到的谱图进行分析，区分HBV患者与正常组的诊断敏感性为92.6%，特异性为77.5%。