SummaryTo explore whether different polyvinylpyrrolidone (PVP) concentrations affect the results of intracytoplasmic sperm injection (ICSI), a prospective study was conducted for 194 couples undergoing 210 ICSI therapy cycles. These cycles were divided into three groups (10, 7 and 5% groups) using the corresponding concentration of PVP for sperm immobilization. The main outcome measures were analyzed. Results indicated that, with a decrease in PVP concentrations, all of the main outcome measures increased. In particular, the high-quality cleavage embryo rate in the 7% group was significantly lower than in the 5% group (P < 0.01), and the cleavage, high-quality cleavage embryo, and high-quality blastocyst rates in the 5% group were significantly higher than those in the 10% group (all P < 0.001). For high-/intermediate-quality semen, all of the main outcome measures were significantly increased with 5% PVP. For the poor-quality semen, only the high-quality cleavage embryo and high-quality blastocyst rates were significantly higher in the 5% group. Therefore, lowering PVP concentrations greatly promoted the development of embryos in ICSI cycles, with an optimal concentration of 5% for ICSI.

中文翻译：

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不同浓度聚乙烯吡咯烷酮对胞浆内单精子注射的影​​响


摘要为了探讨不同的聚乙烯吡咯烷酮 (PVP) 浓度是否影响胞浆内单精子注射 (ICSI) 的结果，对 194 对接受 210 个 ICSI 治疗周期的夫妇进行了一项前瞻性研究。这些循环被分为三组（10%、7% 和 5% 组），使用相应浓度的 PVP 进行精子固定。对主要结果指标进行了分析。结果表明，随着 PVP 浓度的降低，所有主要结果指标均有所增加。特别是7%组的优质卵裂胚胎率明显低于5%组（磷< 0.01），5%组的卵裂率、优质卵裂胚率、优质囊胚率均显着高于10%组（均磷< 0.001）。对于高/中质量精液，5% PVP 后所有主要结果指标均显着提高。对于劣质精液，只有5%组的优质卵裂胚胎和优质囊胚率明显较高。因此，降低PVP浓度极大地促进了ICSI周期中胚胎的发育，ICSI的最佳浓度为5%。