SummarySpontaneous blastocyst collapse during in vitro embryo development has been suggested as a novel marker of embryo quality. Therefore, the aim of this multicentre study was to carry out a retrospective multicentre analysis to investigate the correlation between blastocyst collapse and pregnancy outcome. Here, 1297 intracytoplasmic sperm injection (ICSI)/in vitro fertilization (IVF) fresh cycles, with an elective single blastocyst transfer (eSET) were included in this study. Embryos were cultured individually in 6.0% CO2, 5.0% O2, 89.0% N2, using single step medium (GTLTM VitroLife, Sweden) or sequential medium (CookTM, Cook Medical, Australia) and selected for transfer using standard morphological criteria. With the use of time-lapse monitoring (TLM), blastocysts were analyzed by measuring the maximum volume reduction and defined as having collapsed, if there was ≥ 50% volume reduction from the expanded blastocyst and the collapse event. Following embryo replacement, each blastocyst was retrospectively allocated to one of two groups (collapsed or not collapsed). Here, 259 blastocysts collapsed once or more during development (19.9%) and the remaining 1038 either contracted minimally or not collapsed (80.1%). A significantly higher ongoing pregnancy rate (OPR) of 51.9% (95% CI 48.9–59.9%) was observed when blastocysts that had not collapsed were replaced compared with cycles in which collapsed blastocysts were transferred 37.5% (95% CI 31.6–43.4%). This study suggests that human blastocysts that collapse spontaneously during development are less likely to implant and generate a pregnancy compared with embryos that do not. Although this is a retrospective study, the results demonstrated the utility of collapse episodes as new marker of embryo selection following eSET at blastocyst stage.

中文翻译：

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囊胚塌陷作为低着床潜力的胚胎标志：一项延时多中心研究


摘要 囊胚自发塌陷体外胚胎发育被认为是胚胎质量的新标志。因此，这项多中心研究的目的是进行回顾性多中心分析，以探讨囊胚塌陷与妊娠结局之间的相关性。第1297章 胞浆内单精子注射（ICSI）/体外本研究包括受精（IVF）新鲜周期和选择性单囊胚移植（eSET）。胚胎在 6.0% CO 中单独培养2 , 5.0% 氧气2 , 89.0% 氮2 ，使用单步培养基（GTL TM VitroLife，瑞典）或顺序培养基（Cook TM ，库克医疗，澳大利亚）并使用标准形态学标准选择进行转移。通过使用延时监测 (TLM)，通过测量最大体积减少来分析囊胚，如果扩张的囊胚和塌陷事件导致体积减少 ≥ 50%，则定义为塌陷。胚胎置换后，每个囊胚被回顾性地分配到两组（塌陷或未塌陷）中的一组。其中，259 个囊胚在发育过程中发生一次或多次塌陷 (19.9%)，其余 1038 个囊胚轻微收缩或未塌陷 (80.1%)。与移植塌陷囊胚的周期相比，更换未塌陷囊胚的持续妊娠率 (OPR) 显着更高，为 51.9%（95% CI 48.9-59.9%）（95% CI 31.6-43.4%）。 ）。这项研究表明，与没有自发塌陷的胚胎相比，在发育过程中自发塌陷的人类囊胚植入并怀孕的可能性更小。 尽管这是一项回顾性研究，但结果证明了崩溃事件作为囊胚期 eSET 后胚胎选择新标志的实用性。