SummaryThe abnormal expression of lncRNAs and miRNAs has been found in the placentas of patients with preeclampsia (PE). Therefore, we determined the role of lncRNA FOXD2-AS1/miR-3127 in trophoblast cells. The expression of lncRNA FOXD2-AS1 was detected by qRT-PCR. The proliferation, migration and invasion ability of trophoblast cells were evaluated using CCK-8, wound healing and transwell assays. The target gene of lncRNA FOXD2-AS1 was determined by StarBase and luciferase reporter assays. Western blotting was used to analyze the expression of matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9). The results showed that FOXD2-AS1 affected trophoblast cell viabilityin vitro, while the expression of miR-3127 was decreased. FOXD2-AS1 silencing decreased the promotion effects on trophoblast cell induced by miR-3127 inhibition. In addition, FOXD2-AS1 and miR-3127 presented the same effect on MMP2 and MMP9 levels. lncRNA FOXD2-AS1 modulated trophoblast cell proliferation, invasion and migration through downregulating miR-3127 expression. Therefore, lncRNA FOXD2-AS1 could act as a latent therapeutic marker in preeclampsia.

中文翻译：

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lncRNA FOXD2-AS1通过靶向miRNA影响滋养层细胞增殖、侵袭和迁移


摘要子痫前期（PE）患者胎盘中发现lncRNA和miRNA的异常表达。因此，我们确定了lncRNA FOXD2-AS1/miR-3127在滋养层细胞中的作用。 qRT-PCR检测lncRNA FOXD2-AS1的表达。使用CCK-8、伤口愈合和transwell实验评估滋养层细胞的增殖、迁移和侵袭能力。 lncRNA FOXD2-AS1 的靶基因通过 StarBase 和荧光素酶报告基因测定确定。采用Western blotting分析基质金属蛋白酶2（MMP2）和基质金属蛋白酶9（MMP9）的表达。结果表明FOXD2-AS1影响滋养层细胞活力体外，而miR-3127的表达降低。 FOXD2-AS1沉默降低了miR-3127抑制对滋养层细胞的促进作用。此外，FOXD2-AS1和miR-3127对MMP2和MMP9水平具有相同的影响。 lncRNA FOXD2-AS1 通过下调 miR-3127 表达来调节滋养层细胞增殖、侵袭和迁移。因此，lncRNA FOXD2-AS1可以作为先兆子痫的潜在治疗标志物。