Abstract

Resveratrol is found in numerous plant-based foods and beverages and is known to have an impact on the cardiovascular system. The aim of this study was to investigate the vasorelaxant effect of resveratrol and its underlying mechanisms by employing an aortic ring assay model. Resveratrol caused relaxation of aortic rings that had been precontracted with phenylephrine in the presence of endothelium or with potassium chloride in endothelium-intact aortic rings. The vasorelaxant effect was decreased in the absence of an endothelium. The mechanisms underlying the vasorelaxant effect of resveratrol were determined through the addition of antagonists. In the presence of the endothelium, indomethacin (a nonselective cyclooxygenase inhibitor), methylene blue (cyclic guanosine monophosphate lowering agent), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, selective soluble guanylate cyclase inhibitor), Nω-nitro-l-arginine methyl ester (L-NAME, nitric oxide synthase inhibitor), tetraethylammonium (TEA, nonselective calcium activator potassium channel blocker), 4-aminopyridine (4-AP, voltage-dependent K⁺ channel blocker), barium chloride (BaCl₂, inwardly rectifying K⁺ channel blocker), glibenclamide (non-specific ATP-sensitive K⁺ channel blocker) and propranolol (β-adrenergic receptor blocker) led to a significant reduction in the vasorelaxation effect induced by resveratrol. Resveratrol was also found to reduce Ca²⁺ release from the sarcoplasmic reticulum and block calcium channels. In conclusion, resveratrol targets multiple signalling pathways for exerting its vasorelaxant effects in the rat aortic ring model in both the presence and absence of endothelium.

Graphic abstract

中文翻译：

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3,5,4'-三羟基-反式-二苯乙烯（白藜芦醇）的血管舒张作用及其潜在机理。

摘要

白藜芦醇存在于多种植物性食品和饮料中，已知会对心血管系统产生影响。这项研究的目的是通过采用主动脉环测定模型来研究白藜芦醇的血管舒张作用及其潜在机制。白藜芦醇导致在存在内皮的情况下与去氧肾上腺素或在完整的内皮的主动脉环中与氯化钾预收缩的主动脉环松弛。在没有内皮的情况下血管舒张作用降低。白藜芦醇的血管舒张作用的基本机制是通过加入拮抗剂来确定的。在内皮存在的情况下，使用吲哚美辛（一种非选择性环加氧酶抑制剂），亚甲基蓝（一种环鸟苷单磷酸降低剂），1H- [1,2,4]恶二唑并[4,3-a]喹喔啉-1-酮（ODQ，1-精氨酸甲酯（L-NAME，一氧化氮合酶抑制剂），四乙铵（TEA，非选择性钙激活剂钾通道阻滞剂），4-氨基吡啶（4-AP，电压依赖性K ⁺通道阻滞剂），氯化钡（BaCl ₂，向内纠正K ⁺通道阻滞剂，格列本脲（非特异性ATP敏感性K ⁺通道阻滞剂）和普萘洛尔（β-肾上腺素能受体阻滞剂）导致白藜芦醇诱导的血管舒张作用显着降低。还发现白藜芦醇可减少Ca ²⁺从肌浆网释放并阻断钙通道。总之，白藜芦醇靶向多种信号通路，在存在和不存在内皮的情况下在大鼠主动脉环模型中发挥其血管舒张作用。

图形摘要