Abstract

Compared to one-dimensional gas chromatography, comprehensive two-dimensional gas chromatography (GC × GC) method development is significantly more complex because more method development choices need to be made and because of the complex interplay of the primary and secondary parameters; the individual dimensions cannot be optimized separately. Also, optimization is restricted by requirements such as the modulation criterion and upper temperature limits of the individual columns. In general, the internal diameter of the primary column is larger than the internal diameter of the secondary column which complicates the optimization and leads to sub-optimal flow settings, column loadability issues and indirectly a reduction of the overall separation efficiency. In this review, papers concerning method development for comprehensive two-dimensional gas chromatography (GC × GC) are discussed and general guidelines are proposed with the focus on selecting the GC × GC instrumental set-up and column-set and optimization of the GC × GC settings.

摘要

与一维气相色谱相比，全面的二维气相色谱（GC×GC）方法开发要复杂得多，因为需要选择更多的方法开发选项，并且主要参数和辅助参数之间存在复杂的相互作用。各个尺寸无法单独优化。同样，优化还受到诸如各个列的调制标准和温度上限之类的要求的限制。通常，一级色谱柱的内径大于二级色谱柱的内径，这会使优化工作复杂化，并导致次优流动设置，色谱柱负载性问题以及间接降低整体分离效率。在这篇评论中，