Abstract

1-Deoxynojirimycin (1-DNJ), a polyhydroxylated alkaloid, is a highly selective and potent glycosidase inhibitor that has garnered great interest as a tool to study cellular recognition and as a potential therapeutic agent. The development of analytical methods for the quantification polyhydroxylated alkaloids in natural products requires a multifaceted approach. Many publications over the past five decades have described analytical methods for this compound. However, recently more advanced techniques have come to prominence for sample extraction, purification, detection, and identification. This review provides an updated, extensive overview of the available methods for the extraction, purification, identification or detection of 1-DNJ. The review highlights different strategies for the design of 1-DNJ detection methods, which we analyzed in light of recent detection data. Finally, we conclude with perspectives on possible strategies for increasing the efficiency of identification and quantification of 1-DNJ in the future.

摘要

1-脱氧野尻霉素 (1-DNJ) 是一种多羟基生物碱，是一种高度选择性和有效的糖苷酶抑制剂，作为研究细胞识别的工具和潜在的治疗剂引起了极大的兴趣。天然产物中多羟基生物碱的定量分析方法的开发需要多方面的方法。过去五年中的许多出版物都描述了该化合物的分析方法。然而，最近更先进的技术在样品提取、纯化、检测和鉴定方面变得突出。本综述提供了对 1-DNJ 提取、纯化、鉴定或检测的可用方法的更新、广泛的概述。该综述强调了设计 1-DNJ 检测方法的不同策略，我们根据最近的检测数据进行了分析。最后，我们总结了未来提高 1-DNJ 识别和量化效率的可能策略的观点。