A stain of Pseudomonas sp. Lphe-2, which could degrade phenanthrene as the main carbon and energy source, was isolated from the aerobic sludge of a coking plant. Then its biodegradation characteristics, whole genome sequence and biodegradation pathway were examined. The Lphe-2 strain exhibited broad-spectrum degradation activities for various polycyclic aromatic hydrocarbons (PAHs), including naphthalene (NAP), phenanthrene (PHE), and pyrene (PYR). Under the optimal conditions, the degradation efficiency of phenanthrene (100 mg/L) is 92.76% on the 7th day, and 2-carboxybenzaldehyde and 1-hydroxy-2-naphthoic acid are the major metabolites found in phenanthrene metabolism. Genomic analysis of Pseudomonas sp. Lphe-2 showed that a total of 3879 genes from the Lphe-2 strain were annotated based on the COG classification, and the genomic information was annotated to 185 metabolic pathways. Based on the intermediate metabolites detected by Gas Chromatography-Mass Spectrometer (GC-MS) and all potential phenanthrene-degrading genes identified by BLAST search, a phenanthrene biodegradation pathway of Lphe-2 strain was proposed. These results suggested that Lphe-2 strain has a good prospect in the bioremediation of PAHs pollution.

中文翻译：

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菲降解细菌假单胞菌的鉴定和基因组功能分析。Lphe-2。

假单胞菌属sp的染色。从焦化厂的好氧污泥中分离出Lphe-2，它可以降解菲作为主要的碳和能源。然后检查了其生物降解特性，全基因组序列和生物降解途径。Lphe-2菌株对包括萘（NAP），菲（PHE）和pyr（PYR）在内的各种多环芳烃（PAH）表现出广谱降解活性。在最佳条件下，菲（100 mg / L）在第7天的降解效率为92.76％，其中2-羧基苯甲醛和1-羟基-2-萘甲酸是菲代谢中的主要代谢产物。假单胞菌sp。的基因组分析。Lphe-2显示，根据COG分类，总共注释了来自Lphe-2菌株的3879个基因，并将基因组信息标注为185个代谢途径。基于气相色谱-质谱仪（GC-MS）检测到的中间代谢物以及通过BLAST搜索鉴定的所有潜在的菲降解基因，提出了Lphe-2菌株的菲生物降解途径。这些结果表明，Lphe-2菌株在PAHs污染的生物修复中具有良好的前景。