LncRNA PTENP1 is a competitive endogenous RNA (ceRNA) involved in decoying miR-106b in multiple diseases. This study investigates the interaction of PTENP1 and miR-106b in cell proliferation, apoptosis and epithelial-mesenchymal transition (EMT) in cervical cancer. The expressions of PTENP1, miR-106b and PTEN were determined in cervical cancer tissues, adjacent normal tissues, cervical cancer cells (HeLa, SiHa, C33A and CasKi) and normal cervical epithelial H8 cells. Up-regulation of PTENP1 and down-regulation of miR-106b were conducted in HeLa and CasKi cells by transfecting cells with corresponding miRNA mimics and inhibitors. Bioinformatics analysis, luciferase reporter assay and RNA-pull down assay were performed to verify the association of miR-106b, PTEN, and PTENP1. Cell growth and cell apoptosis were determined by CCK-8 and flow cytometry analysis. It was found that the expressions of PTENP1 and PTEN were up-regulated and that of miR-106b were down-regulated in cervical cancer tissues and cells. PTENP1 localized in cytoplasm and competitively bound to miR-106b. Up-regulation of PTENP1 and down-regulation of miR-106b contributed to increased expressions of PTEN and E-cadherin. Decreased expression of miR-106b, ZEB1, Snail and Vimentin, resulted in inhibiting cell proliferation and promoting cell apoptosis. Over-expression of PTENP1 and miR-106b accelerated cell proliferation and slowed down cell apoptosis. miR-106b inhibited the expression of PTEN. Our results suggest that LncRNA PTENP1 inhibits cervical cancer progression by competitively binding to miR-106b, leading to promote PTEN expression, inhibit cell proliferation and EMT and induce cell apoptosis in cervical cancer cells.

中文翻译：

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LncRNA PTENP1通过抑制miR-106b抑制子宫颈癌的进展。

LncRNA PTENP1是一种竞争性内源RNA（ceRNA），涉及多种疾病中miR-106b的诱变。这项研究调查了PTENP1和miR-106b在宫颈癌的细胞增殖，凋亡和上皮-间质转化（EMT）中的相互作用。在宫颈癌组织，邻近的正常组织，宫颈癌细胞（HeLa，SiHa，C33A和CasKi）和正常宫颈上皮H8细胞中检测PTENP1，miR-106b和PTEN的表达。通过用相应的miRNA模拟物和抑制剂转染细胞，在HeLa和CasKi细胞中进行PTENP1的上调和miR-106b的下调。进行了生物信息学分析，荧光素酶报告基因分析和RNA下拉分析，以验证miR-106b，PTEN和PTENP1的关联。通过CCK-8和流式细胞仪分析确定细胞生长和细胞凋亡。发现在宫颈癌组织和细胞中PTENP1和PTEN的表达上调，而miR-106b的表达下调。PTENP1定位在细胞质中，与miR-106b竞争结合。PTENP1的上调和miR-106b的下调有助于PTEN和E-钙粘蛋白的表达增加。miR-106b，ZEB1，Snail和波形蛋白的表达降低，导致抑制细胞增殖和促进细胞凋亡。PTENP1和miR-106b的过度表达可促进细胞增殖并减缓细胞凋亡。miR-106b抑制PTEN的表达。我们的结果表明，LncRNA PTENP1通过竞争性结合miR-106b抑制宫颈癌的进展，从而促进PTEN表达，