当前位置: X-MOL 学术Comp. Cytogenet. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Molecular-cytogenetic analysis of diploid wheatgrass Thinopyrum bessarabicum (Savul. and Rayss) A. Löve.
Comparative Cytogenetics ( IF 1.0 ) Pub Date : 2019-12-03 , DOI: 10.3897/compcytogen.v13i4.36879
Ekaterina D Badaeva 1, 2 , Sergei A Surzhikov 2 , Alexander V Agafonov 3
Affiliation  

Thinopyrum bessarabicum (T. Săvulescu & T. Rayss, 1923) A. Löve, 1980 is diploid (2n=2x=14, JJ or EbEb), perennial self-fertilizing rhizomatous maritime beach grass, which is phylogenetically close to another diploid wheatgrass species, Agropyron elongatum (N. Host, 1797) P. de Beauvois, 1812. The detailed karyotype of Th. bessarabicum was constructed based on FISH with six DNA probes representing 5S and 45S rRNA gene families and four tandem repeats. We found that the combination of pAesp_SAT86 (= pTa-713) probe with pSc119.2 or pAs1/ pTa-535 allows the precise identification of all J-genome chromosomes. Comparison of our data with the results of other authors showed that karyotypically Th. bessarabicum is distinct from A. elongatum. On the other hand, differences between the J-genome chromosomes of Th. bessarabicum and the chromosomes of hexaploid Th. intermedium (N. Host, 1797) M. Barkworth & D.R. Dewey, 1985 and decaploid Th. ponticum (J. Podpěra, 1902) Z.-W. Liu & R.-C. Wang, 1993 in the distribution of rDNA loci and hybridization patterns of pSc119.2 and pAs1 probes could be an indicative of (1) this diploid species was probably not involved in the origin of these polyploids or (2) it could has contributed the J-genome to Th. intermedium and Th. ponticum, but it was substantially modified over the course of speciation.

中文翻译:

二倍体小麦草芥菜(Savul。and Rayss)A。Löve的分子细胞遗传学分析。

s草(T.Săvulescu&T.Rayss,1923)A.Löve,1980是二倍体(2n = 2x = 14,JJ或EbEb),多年生自肥根茎海生沙滩草,在系统发育上与另一个二倍体小麦草物种接近。 ,Agropyron elongatum(N. Host,1797)P. de Beauvois,1812。基于FISH构建的bessarabicum具有六个代表5S和45S rRNA基因家族的DNA探针和四个串联重复序列。我们发现pAesp_SAT86(= pTa-713)探针与pSc119.2或pAs1 / pTa-535的组合可以精确鉴定所有J基因组染色体。我们的数据与其他作者的结果进行比较表明,核型。笔杆不同于长曲霉。另一方面,Th的J基因组染色体之间的差异。s和六倍体Th的染色体。媒介(N. Host,1797)M. Barkworth&DR Dewey,1985和decaploid Th。ponticum(J.Podpěra,1902)Z.-W. Liu&R.-C. Wang,1993年,在rDNA基因座的分布以及pSc119.2和pAs1探针的杂交模式中可能指示(1)该二倍体物种可能不参与这些多倍体的起源,或者(2)可能对-基因组到Th。中等和Th。ponticum,但在物种形成过程中进行了重大修改。2和pAs1探针可能表明(1)该二倍体物种可能不参与这些多倍体的起源,或者(2)它可能为Th贡献了J基因组。中间和Th。ponticum,但在物种形成过程中进行了重大修改。2和pAs1探针可能表明(1)该二倍体物种可能不参与这些多倍体的起源,或者(2)它可能为Th贡献了J基因组。中间和Th。ponticum,但在物种形成过程中进行了重大修改。
更新日期:2019-12-03
down
wechat
bug