Non-small cell lung cancer (NSCLC) is the leading cause of cancer mortality worldwide. Distant metastasis is thought to be one of the most important factors responsible for the failure of NSCLC therapy. MicroRNA-7-5p (miR-7-5p) has been demonstrated to be a tumor suppressor in breast cancer, hepatocarcinoma, prostate cancer and glioblastoma multiforme (GBM). However, its role in NSCLC is still not fully understood. This study evaluated the role of miR-7-5p in the progression of NSCLC and explored the underlying mechanism. The quantitative real-time PCR (qPCR), MTT, migration and invasion assays were used to evaluate the effects of miR-7-5p on the proliferation, migration and invasion of A549 and SPCA-1 cells. A tumor xenograft model was created to determine the effects of miR-7-5p on metastasis in vivo. The dual-luciferase reporter gene, neuro-oncological ventral antigen 2 (NOVA2) overexpression and western blotting assays were performed to explore the underlying mechanism. MiR-7-5p is downregulated in NSCLC tissues and lung cancer cell lines. It suppresses proliferation, migration, invasion and EMT marker expression in vitro and in vivo. Further study showed that miR-7-5p suppresses tumor metastasis of NSCLC by targeting NOVA2. Overexpression of NOVA2 attenuates the miR-7-5p-mediated inhibitory effect on lung cancer cells. MiR-7-5p suppresses NSCLC metastasis. Targeting miR-7-5p may contribute to the success of NSCLC therapy.

中文翻译：

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MiR-7-5p 通过靶向 NOVA2 抑制非小细胞肺癌的肿瘤转移。

非小细胞肺癌（NSCLC）是全球癌症死亡的主要原因。远处转移被认为是导致NSCLC治疗失败的最重要因素之一。MicroRNA-7-5p (miR-7-5p) 已被证明是乳腺癌、肝癌、前列腺癌和多形性胶质母细胞瘤 (GBM) 中的肿瘤抑制因子。然而，其在 NSCLC 中的作用仍未完全了解。本研究评估了 miR-7-5p 在 NSCLC 进展中的作用，并探讨了其潜在机制。采用定量实时PCR（qPCR）、MTT、迁移和侵袭试验评估miR-7-5p对A549和SPCA-1细胞增殖、迁移和侵袭的影响。建立肿瘤异种移植模型以确定 miR-7-5p 对体内转移的影响。双荧光素酶报告基因，进行神经肿瘤腹侧抗原 2 (NOVA2) 过表达和蛋白质印迹测定以探索潜在机制。MiR-7-5p 在 NSCLC 组织和肺癌细胞系中下调。它在体外和体内抑制增殖、迁移、侵袭和 EMT 标志物的表达。进一步的研究表明，miR-7-5p 通过靶向 NOVA2 抑制 NSCLC 的肿瘤转移。NOVA2 的过表达减弱了 miR-7-5p 介导的对肺癌细胞的抑制作用。MiR-7-5p 抑制 NSCLC 转移。靶向 miR-7-5p 可能有助于 NSCLC 治疗的成功。体外和体内侵袭和 EMT 标志物的表达。进一步的研究表明，miR-7-5p 通过靶向 NOVA2 抑制 NSCLC 的肿瘤转移。NOVA2 的过表达减弱了 miR-7-5p 介导的对肺癌细胞的抑制作用。MiR-7-5p 抑制 NSCLC 转移。靶向 miR-7-5p 可能有助于 NSCLC 治疗的成功。体外和体内侵袭和 EMT 标志物的表达。进一步的研究表明，miR-7-5p 通过靶向 NOVA2 抑制 NSCLC 的肿瘤转移。NOVA2 的过表达减弱了 miR-7-5p 介导的对肺癌细胞的抑制作用。MiR-7-5p 抑制 NSCLC 转移。靶向 miR-7-5p 可能有助于 NSCLC 治疗的成功。