A method for analysis of psychotropic drugs in urine is investigated using a novel single-drop liquid-liquid-liquid microextraction (SDLLLME) apparatus as an electrospray emitter in ion mobility spectrometry (IMS). In this method, ketamine and pethidine are back-extracted into the acceptor phase (water and methanol) from the water and methanol immiscible organic phase. Sensitivity of extraction is improved as it does not require further methanol-adding procedure. Factors affecting the extraction of psychotropic drugs are characterized, including organic solvent type, extraction time, and concentration of NaOH/Ac in the donor/acceptor phase. The best extraction sensitivity is obtained with 600 μL dodecane as the organic phase, 30 minutes extraction time, and 10 mL donor phase with 0.01 M sodium hydroxide (NaOH) and 3 μL acceptor phase with 0.5 M acetic acid (Ac). Using this method, the two analytes can be extracted and analyzed simultaneously, showing this method is valuable for field application.

中文翻译：

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离子淌度-电喷雾耦合在线单滴液-液微萃取的离子迁移谱法对尿液样品中的精神药物进行预浓缩和测定。

使用新型单滴液-液-液微萃取（SDLLLME）装置作为离子迁移谱（IMS）中的电喷雾发射器，研究了一种分析尿液中精神药物的方法。在这种方法中，氯胺酮和哌啶从与水和甲醇不混溶的有机相反萃取到受体相（水和甲醇）中。萃取的灵敏度得以提高，因为它不需要进一步的甲醇添加程序。表征影响精神药物提取的因素，包括有机溶剂类型，提取时间和供体/受体相中NaOH / Ac的浓度。使用600μL十二烷作为有机相，30分钟的萃取时间，10 mL供体相，0.01 M氢氧化钠（NaOH）和3μL受体相，0可获得最佳的萃取灵敏度。5 M乙酸（Ac）。使用这种方法，可以同时提取和分析两种分析物，表明该方法对于现场应用很有价值。