The study describes an “open” method for concentrating anionic bilayer lipid vesicles (liposomes) locally by electrostatically binding them in a complex with cationic polymers. This method is implemented by mixing premade solutions of liposomes and polymers, which significantly reduces the time and cost of obtaining multiliposomal complexes. Binding of liposomes with cationic linear polymers, latexes, and star polymers does not solve the problem of obtaining multiliposomal complexes: the size of such complexes cannot be controlled, and these variants fail to ensure the integrity of bound liposomes or result in complexes with a minimal amount of liposomes. The best results are demonstrated by complexes of anionic liposomes and polystyrene nanoparticles with grafted cationic chains (spherical polycationic brushes). Each brush can bind several dozen liposomes, which retain their integrity after being adsorbed on the surface of the brush. Such complexes do not dissociate into the initial components either in physiological saline with [NaCl] = 0.15 mol/L or in the presence of significant amounts of protein. The use of liposomes with different fillers (different liposomal “compartments”) makes it possible to obtain multiliposomal complexes with the desired fraction of substances encapsulated in the liposomes. The proposed approach is of interest in terms of obtaining multiliposomal complexes for concentration, compartmentalization, and subsequent controlled release of drugs.

这项研究描述了一种“开放式”方法，该方法可通过将阴离子双层脂质囊泡（脂质体）与阳离子聚合物复合形成一种静电结合，从而局部浓缩它们。该方法是通过混合脂质体和聚合物的预制溶液来实现的，这大大减少了获得多脂质体复合物的时间和成本。脂质体与阳离子线性聚合物，胶乳和星形聚合物的结合不能解决获得多脂质体复合物的问题：此类复合物的大小无法控制，并且这些变体无法确保结合的脂质体的完整性或导致复合物的含量极低脂质体的量。阴离子脂质体和聚苯乙烯纳米颗粒与接枝的阳离子链（球形聚阳离子刷）的复合物证明了最佳结果。每个刷子可以结合数十个脂质体，这些脂质体在被吸附在刷子表面后仍保持其完整性。在[NaCl] = 0.15 mol / L的生理盐水中或存在大量蛋白质的情况下，此类复合物均不会解离为初始成分。将脂质体与不同的填充剂一起使用（不同的脂质体“区室”）可以得到具有脂质体中所需比例的物质的多脂质体复合物。就获得用于脂质的浓缩，分隔和随后的药物控释的多脂质体复合物而言，所提出的方法是令人感兴趣的。在[NaCl] = 0.15 mol / L的生理盐水中或存在大量蛋白质的情况下，此类复合物均不会解离为初始成分。将脂质体与不同的填充剂一起使用（不同的脂质体“区室”）可以得到具有脂质体中所需比例的物质的多脂质体复合物。就获得用于脂质的浓缩，分隔和随后的药物控释的多脂质体复合物而言，所提出的方法是令人感兴趣的。在[NaCl] = 0.15 mol / L的生理盐水中或存在大量蛋白质的情况下，此类复合物均不会解离为初始成分。将脂质体与不同的填充剂一起使用（不同的脂质体“区室”）可以得到具有脂质体中所需比例的物质的多脂质体复合物。就获得用于脂质的浓缩，分隔和随后的药物控释的多脂质体复合物而言，所提出的方法是令人感兴趣的。