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Maize WI5 encodes an endo-1,4-β-xylanase required for secondary cell wall synthesis and water transport in xylem.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-03-04 , DOI: 10.1111/jipb.12923 Xiaojiao Hu 1 , Yang Cui 1 , Xiaomin Lu 1 , Weibin Song 1 , Lei Lei 1 , Jinjie Zhu 1 , Jinsheng Lai 1 , Lizhu E 1 , Haiming Zhao 1
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-03-04 , DOI: 10.1111/jipb.12923 Xiaojiao Hu 1 , Yang Cui 1 , Xiaomin Lu 1 , Weibin Song 1 , Lei Lei 1 , Jinjie Zhu 1 , Jinsheng Lai 1 , Lizhu E 1 , Haiming Zhao 1
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Water transport from roots to leaves through xylem is important for plant growth and development. Defects in water transport can cause drought stress, even when there is adequate water in the soil. Here, we identified the maize (Zea mays) wilty5 (wi5) mutant, which exhibits marked dwarfing and leaf wilting throughout most of its life cycle under normal growth conditions. wilty5 seedlings exhibited lower xylem conductivity and wilted more rapidly under drought, NaCl, and high temperature treatments than wild‐type plants. Map‐based cloning revealed that WI5 encodes an active endo‐1,4‐β‐xylanase from glycosyl dehydration family 10, which mainly functions in degrading and reorganizing cell wall xylan. Reverse‐transcription polymerase chain reaction and β‐glucuronidase assays revealed that WI5 is highly expressed in stems, especially in internodes undergoing secondary wall assembly. RNA sequencing suggested that WI5 plays a unique role in internode growth. Immunohistochemistry and electron microscopy confirmed that wi5 is defective in xylan deposition and secondary cell wall thickening. Lignin deposition and xylan content were markedly reduced in wi5 compared to the wild‐type plants. Our results suggest that WI5 functions in xylem cell wall thickening through its xylanase activity and thereby regulates xylem water transport, the drought stress response, and plant growth in maize.
中文翻译：
玉米 WI5 编码次生细胞壁合成和木质部水分运输所需的内切 1,4-β-木聚糖酶。
水分通过木质部从根部输送到叶子对于植物生长和发育很重要。即使土壤中有足够的水，水运输的缺陷也会导致干旱胁迫。在这里，我们鉴定了玉米 ( Zea mays ) wilty5 ( wi5 ) 突变体，该突变体在正常生长条件下的大部分生命周期中表现出明显的矮化和叶片枯萎。与野生型植物相比，wilty5幼苗的木质部电导率较低，并且在干旱、氯化钠和高温处理下枯萎得更快。基于图谱的克隆显示，WI5编码来自糖基脱水家族10的活性内切-1,4-β-木聚糖酶，其主要功能是降解和重组细胞壁木聚糖。逆转录聚合酶链反应和β-葡萄糖醛酸酶测定表明，WI5在茎中高表达，特别是在正在进行次生壁组装的节间。RNA测序表明WI5在节间生长中发挥着独特的作用。免疫组织化学和电子显微镜证实wi5在木聚糖沉积和次生细胞壁增厚方面存在缺陷。与野生型植物相比，wi5中的木质素沉积和木聚糖含量显着减少。我们的结果表明，WI5通过其木聚糖酶活性在木质部细胞壁增厚中发挥作用，从而调节玉米的木质部水分运输、干旱胁迫反应和植物生长。
更新日期：2020-03-04
中文翻译：
玉米 WI5 编码次生细胞壁合成和木质部水分运输所需的内切 1,4-β-木聚糖酶。
水分通过木质部从根部输送到叶子对于植物生长和发育很重要。即使土壤中有足够的水，水运输的缺陷也会导致干旱胁迫。在这里，我们鉴定了玉米 ( Zea mays ) wilty5 ( wi5 ) 突变体，该突变体在正常生长条件下的大部分生命周期中表现出明显的矮化和叶片枯萎。与野生型植物相比，wilty5幼苗的木质部电导率较低，并且在干旱、氯化钠和高温处理下枯萎得更快。基于图谱的克隆显示，WI5编码来自糖基脱水家族10的活性内切-1,4-β-木聚糖酶，其主要功能是降解和重组细胞壁木聚糖。逆转录聚合酶链反应和β-葡萄糖醛酸酶测定表明，WI5在茎中高表达，特别是在正在进行次生壁组装的节间。RNA测序表明WI5在节间生长中发挥着独特的作用。免疫组织化学和电子显微镜证实wi5在木聚糖沉积和次生细胞壁增厚方面存在缺陷。与野生型植物相比，wi5中的木质素沉积和木聚糖含量显着减少。我们的结果表明，WI5通过其木聚糖酶活性在木质部细胞壁增厚中发挥作用，从而调节玉米的木质部水分运输、干旱胁迫反应和植物生长。
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