Fundamentals of single particle analysis in biomatrices by laser ablation-inductively coupled plasma mass spectrometry,Journal of Analytical Atomic Spectrometry

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Fundamentals of single particle analysis in biomatrices by laser ablation-inductively coupled plasma mass spectrometry
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2020-03-04 , DOI: 10.1039/d0ja00003e
Dino Metarapi _{1,

2,

3,

4,

5} , Johannes T. van Elteren _{1,

2,

3,

4}

Affiliation

True metal nanoparticle (NP) analysis directly in solid biomatrices, involving sizing and counting, using the recently introduced laser ablation-single particle-inductively coupled plasma mass spectrometry (LA-SP-ICPMS) technique, is prone to produce erratic results when the instrument is not set up correctly and non-optimal operational conditions are used. This work offers deeper insights into the analysis of NPs by LA-SP-ICPMS, based on modeling the chain of events from NP release to LA cell washout to ICPMS detection as a function of the biomatrix characteristics, instrumental specifications and variable operational settings. By processing the computational data via an “outlier” filter to differentiate between metal NPs and dissolved metal, guidelines were established for instrumental setup and operation, assuming the measurement of homogeneously distributed metal NPs and dissolved metal, and using a laser fluence ≤1 J cm⁻² to circumvent NP degradation. An online app (http://193.2.14.6:9988/webapps/home/) was developed, based on the model, to predict the SP analysis capabilities of arbitrary LA-ICPMS setups using a custom detectability criterion.

中文翻译：

激光烧蚀-电感耦合等离子体质谱法对生物基质中单颗粒分析的基础知识

使用最近推出的激光烧蚀-单颗粒电感耦合等离子体质谱法（LA-SP-ICPMS）技术，直接在固体生物基质中进行真正的金属纳米颗粒（NP）分析，包括进行尺寸计算和计数，当该仪器出现测量结果时，往往会产生不稳定的结果设置不正确，并且使用了非最佳操作条件。这项工作根据生物基质特性，仪器规格和可变操作设置的函数，对从NP释放到LA细胞洗脱到ICPMS检测的事件链进行建模，为通过LA-SP-ICPMS分析NP提供了更深入的见解。通过处理计算数据经由区分金属NP和溶解金属的“异常值”过滤器，建立了仪器设置和操作的准则，假设测量的是均匀分布的金属NP和溶解金属，并使用≤1 J cm ^-2的激光通量来规避NP的降解。基于该模型，开发了一个在线应用程序（http://193.2.14.6:9988/webapps/home/），以使用自定义可检测性标准预测任意LA-ICPMS设置的SP分析功能。

更新日期：2020-04-24

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