Enzymatic preparation of a low-molecular-weight polysaccharide rich in uronic acid from the seaweed Laminaria japonica and evaluation of its hypolipidemic effect in mice.,Food & Function

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Enzymatic preparation of a low-molecular-weight polysaccharide rich in uronic acid from the seaweed Laminaria japonica and evaluation of its hypolipidemic effect in mice.
Food & Function ( IF 5.1 ) Pub Date : 2020/03/04 , DOI: 10.1039/c9fo02994j
Nannan Li ₁ , Xiaodan Fu ₁ , Mengshi Xiao ₁ , Xinyi Wei ₁ , Min Yang ₁ , Zhemin Liu ₁ , Haijin Mou ₁

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Here, we describe a method combining thermo-acid pretreatment and alginate lyase hydrolysis to prepare a low-molecular-weight polysaccharide from the seaweed Laminaria japonica (SP). The in vitro results showed that SP displayed obvious absorption of oil (2.95 g g-1) and cholesterol (21.87 g g-1 at pH 2.0). In addition, the in vivo assessment of SP-related anti-obesity effects in C57BL/6J mice fed a high-fat diet and treated with SP for 8 weeks revealed that SP significantly reduced weight gain and lipid accumulation in white adipose and liver tissues, improved serum lipid profiles, and ameliorated intestinal damage. Moreover, SP activated the AMP-activated protein kinase pathway in liver tissues, downregulated sterol regulatory element-binding protein and fatty acid synthase, and suppressed lipid synthesis. These findings indicated that SP extracted from L. japonica might represent a potent functional food exhibiting anti-obesity effects.

中文翻译：

海藻海带中富含糖醛酸的低分子量多糖的酶法制备及其对小鼠的降血脂作用评价。

在这里，我们描述了一种结合热酸预处理和海藻酸裂解酶水解的方法，从海藻海带（SP）中制备低分子量多糖。体外结果表明，SP对油（2.95 g g-1）和胆固醇（21.87 g g-1，pH 2.0）有明显的吸收作用。此外，对喂食高脂饮食并用 SP 治疗 8 周的 C57BL/6J 小鼠进行 SP 相关抗肥胖作用的体内评估表明，SP 显着降低了体重增加和白色脂肪和肝组织中的脂质积累，改善血清脂质状况，并减轻肠道损伤。此外，SP激活肝组织中的AMP激活蛋白激酶途径，下调甾醇调节元件结合蛋白和脂肪酸合酶，并抑制脂质合成。这些发现表明，从 L. japonica 中提取的 SP 可能是一种具有抗肥胖作用的有效功能食品。

更新日期：2020-03-27

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