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A DNA tweezers-actuated regenerated DNAzyme nanoreactor: a fluorescence sensor for miRNA and adenosine detection
Analytical Methods ( IF 2.7 ) Pub Date : 2020/03/04 , DOI: 10.1039/d0ay00142b Xueying Chen 1, 2, 3, 4, 5 , Xinrui Fu 1, 2, 3, 4, 5 , Yuanyu Wu 1, 2, 3, 4, 5 , Yifan Jin 1, 2, 3, 4, 5 , Wei Li 1, 2, 3, 4, 5
Analytical Methods ( IF 2.7 ) Pub Date : 2020/03/04 , DOI: 10.1039/d0ay00142b Xueying Chen 1, 2, 3, 4, 5 , Xinrui Fu 1, 2, 3, 4, 5 , Yuanyu Wu 1, 2, 3, 4, 5 , Yifan Jin 1, 2, 3, 4, 5 , Wei Li 1, 2, 3, 4, 5
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A DNA tweezers-actuated regenerated DNAzyme nanoreactor has been constructed. DNA tweezers that can be reversibly switched between the open and closed states were designed and served as a scaffold to regulate the DNAzyme. In the original open state, the split DNAzymes were spatially separated at the two tweezers arms without catalytic efficiency. When fuel strands were added, the tweezers converted to a closed state that holds the two tweezers arms close together, forming an integral DNAzyme with an active secondary structure. Then the active DNAzyme could catalyze the substrate cleavage with the aid of cofactors, generating detectable and amplified signals. Especially, the DNA tweezers could switch back to the original open state when fresh set strands were added, resulting in the separation of the split DNAzymes. The regulation of the DNAzyme nanoreactor was achieved for four cycles, and the DNAzyme nanoreactor could be regenerated for consecutive target detection. Moreover, as a fluorescence biosensor, the proposed DNAzyme nanoreactor has been applied in miRNA and adenosine detection, exhibiting excellent sensitivity, specificity, and perfect capability for practical application. Thus, it provides a reusable platform for clinical detection and could be further applied in clinical early diagnosis and treatment.
中文翻译：
DNA镊子驱动的再生DNAzyme纳米反应器：用于miRNA和腺苷检测的荧光传感器
已构建了由DNA镊子驱动的再生DNAzyme纳米反应器。设计了可以在打开状态和关闭状态之间可逆切换的DNA镊子，并用作调节DNAzyme的支架。在原始的打开状态下，分裂的DNA酶在两个镊子臂上在空间上分离，而没有催化效率。当添加燃料束时，镊子转换为闭合状态，将两个镊子臂保持在一起，形成具有活性二级结构的完整DNAzyme。然后，活性DNA核酶可以借助辅因子催化底物裂解，产生可检测和放大的信号。特别是，当添加新鲜的固定链时，DNA镊子可能会切换回原始的打开状态，从而导致分裂的DNAzyme分离。DNAzyme纳米反应器的调节达到四个周期，并且DNAzyme纳米反应器可以再生用于连续的靶标检测。此外，作为荧光生物传感器，提出的DNAzyme纳米反应器已应用于miRNA和腺苷检测，具有极好的灵敏度，特异性和完美的实际应用能力。因此，它为临床检测提供了可重用的平台，并可以进一步应用于临床早期诊断和治疗。
更新日期：2020-03-27
中文翻译：
DNA镊子驱动的再生DNAzyme纳米反应器：用于miRNA和腺苷检测的荧光传感器
已构建了由DNA镊子驱动的再生DNAzyme纳米反应器。设计了可以在打开状态和关闭状态之间可逆切换的DNA镊子，并用作调节DNAzyme的支架。在原始的打开状态下，分裂的DNA酶在两个镊子臂上在空间上分离，而没有催化效率。当添加燃料束时，镊子转换为闭合状态，将两个镊子臂保持在一起，形成具有活性二级结构的完整DNAzyme。然后，活性DNA核酶可以借助辅因子催化底物裂解，产生可检测和放大的信号。特别是，当添加新鲜的固定链时，DNA镊子可能会切换回原始的打开状态，从而导致分裂的DNAzyme分离。DNAzyme纳米反应器的调节达到四个周期，并且DNAzyme纳米反应器可以再生用于连续的靶标检测。此外，作为荧光生物传感器，提出的DNAzyme纳米反应器已应用于miRNA和腺苷检测，具有极好的灵敏度，特异性和完美的实际应用能力。因此，它为临床检测提供了可重用的平台，并可以进一步应用于临床早期诊断和治疗。
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