In the present study, an original electrode fabrication approach was devised to create a label free sensitive electrochemical aptasensor for the detection of Homocysteine (Hcy) (Homocysteine signal was used for detection). To bind certain targets, synthetic oligonucleotides used as aptamers (APs) were specifically selected. Aptamers are substitutes for antibodies for analytical devices because of their sensitivity and high affinity. In this study, Hcy-Binding-Aptamer (HBA) was grafted onto the surface of Au nanoparticles/Glassy Carbon Electrode (Au/GCE) in order to create an aptasensor. The effects of buffer concentration, buffer type, interaction time, and aptamer concentration were investigated and optimized. In addition, Differential Pulse Voltammetry (DPV) was implemented to identify homocysteine. Favorable performance was achieved at a detection limit of 0.01 μM (S/N = 3) and linear range 0.05-20.0 μM. Furthermore, the fabricated aptasensor displayed desirable stability and reproducibility. The developed electrochemical aptasensor was found to have reasonable selectivity for the detection of homocysteine in the presence of cysteine and methionine. Analysis of real samples showed good ability of the proposed homocysteine biosensor to provide sensitive, quick, easy, and cost effective measurement of homocysteine in human blood serum and urine samples.

中文翻译：

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基于金纳米颗粒的高灵敏度检测高半胱氨酸的无标记适体传感器。

在本研究中，设计了一种原始的电极制造方法来创建用于检测同型半胱氨酸（Hcy）的无标记的灵敏电化学适体传感器（使用同型半胱氨酸信号进行检测）。为了结合某些靶标，专门选择了用作适体（AP）的合成寡核苷酸。适体由于其灵敏性和高亲和力而可替代分析仪器的抗体。在这项研究中，Hcy结合适体（HBA）被嫁接到Au纳米颗粒/玻璃碳电极（Au / GCE）的表面上，以创建一个适体传感器。研究并优化了缓冲液浓度，缓冲液类型，相互作用时间和适体浓度的影响。此外，实施差分脉冲伏安法（DPV）来鉴定高半胱氨酸。在0.01μM（S / N = 3）的检出限和0.05-20.0μM的线性范围内实现了良好的性能。此外，制造的适体传感器显示出所需的稳定性和可再现性。发现开发的电化学适体传感器在存在半胱氨酸和蛋氨酸的情况下对高半胱氨酸的检测具有合理的选择性。对真实样品的分析表明，所提出的同型半胱氨酸生物传感器具有良好的能力，能够提供灵敏，快速，简便且经济高效的人血清和尿液样品中同型半胱氨酸测量方法。发现开发的电化学适体传感器在存在半胱氨酸和蛋氨酸的情况下对高半胱氨酸的检测具有合理的选择性。对真实样品的分析表明，所提出的同型半胱氨酸生物传感器具有良好的能力，能够提供灵敏，快速，简便且经济高效的人血清和尿液样品中同型半胱氨酸测量方法。发现开发的电化学适体传感器在存在半胱氨酸和蛋氨酸的情况下对高半胱氨酸的检测具有合理的选择性。对真实样品的分析表明，所提出的同型半胱氨酸生物传感器具有良好的能力，能够提供灵敏，快速，简便且经济高效的人血清和尿液样品中同型半胱氨酸测量方法。