Low-level contamination of food and feed by deoxynivalenol (DON) is unavoidable.

We investigated the effects of subclinical treatment with DON, and supplementation with probiotic yeast Saccharomyces cerevisiae boulardii I1079 as a preventive strategy in piglets.

Thirty-six animals were randomly assigned to either a control diet, a diet contaminated with DON (3 mg/kg), a diet supplemented with yeast (4 × 10⁹ CFU/kg), or a DON-contaminated diet supplemented with yeast, for four weeks. Plasma and tissue samples were collected for biochemical analysis, ¹H-NMR untargeted metabolomics, and histology.

DON induced no significant modifications in biochemical parameters. However, lesion scores were higher and metabolomics highlighted alterations of amino acid and 2-oxocarboxylic acid metabolism. Administering yeast affected aminoacyl-tRNA synthesis and amino acid and glycerophospholipid metabolism. Yeast supplementation of piglets exposed to DON prevented histological alterations, and partial least square discriminant analysis emphasised similarity between the metabolic profiles of their plasma and that of the control group. The effect on liver metabolome remained marginal, indicating that the toxicity of the mycotoxin was not eliminated.

These findings show that the ¹H-NMR metabolomics profile is a reliable biomarker to assess subclinical exposure to DON, and that supplementation with S. cerevisiae boulardii increases the resilience of piglets to this mycotoxin.

脱氧雪茄烯醇（DON）不可避免地对食品和饲料造成少量污染。

我们研究了DON亚临床治疗和补充的作用益生菌酵母酿酒酵母布拉I1079在仔猪预防战略。

将36只动物随机分为对照饮食，受DON污染的饮食（3 mg / kg），补充有酵母的饮食（4×10 ⁹  CFU / kg）或受DON污染的饮食和酵母，四个星期。收集血浆和组织样品进行生化分析，¹ H-NMR非靶向代谢组学和组织学。

DON在生化参数上没有引起显着改变。但是，病变评分更高，代谢组学突出显示了氨基酸和2-氧代羧酸代谢的改变。施用酵母影响氨基酰基-tRNA的合成以及氨基酸和甘油磷脂代谢。暴露于DON的仔猪的酵母补充可防止组织学改变，偏最小二乘判别分析强调其血浆和对照组的代谢曲线相似。对肝脏代谢组的影响仍然很小，表明霉菌毒素的毒性并未消除。

这些发现表明¹ H-NMR代谢组学谱是评估亚临床暴露于DON的可靠生物标志物，补充啤酒糖酵母有助于提高仔猪对这种霉菌毒素的抵抗力。