Quantitation of polymeric-microneedle-delivered HA15 in tissues using liquid chromatography-tandem mass spectrometry.,Journal of Pharmaceutical and Biomedical Analysis

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Quantitation of polymeric-microneedle-delivered HA15 in tissues using liquid chromatography-tandem mass spectrometry.
Journal of Pharmaceutical and Biomedical Analysis ( IF 3.1 ) Pub Date : 2020-03-04 , DOI: 10.1016/j.jpba.2020.113230
Parbeen Singh ₁ , Xiliu Zeng ₂ , Xiaowu Chen ₃ , Yikun Yang ₄ , Yongli Chen ₁ , Shufen Cui ₂ , Andrew Carrier ₅ , Ken Oakes ₆ , Tiangang Luan ₇ , Xu Zhang ₅

Affiliation

A rapid and sensitive liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of HA15, an emerging anticancer compound targeting GSPA5/BIP delivered by dissolvable polymeric microneedles. The linear range of quantification for HA15 was 2.5-1000 ng/ml in plasma and tissue homogenate and the limit of detection and lower limit of quantification are 1 and 2.5 ng/ml, respectively. The inter- and intra-day accuracy and precision were within the acceptable range. HA15 was extracted from mouse plasma and organs using protein precipitation and using dabrafenib as an internal standard and the drug was stable under relevant analytical conditions. The method was used to analyze drug loading, dissolution in vitro, and release ex vivo from dissolvable polymeric microneedles and used to compare these materials to subcutaneous injection for the tissue distribution in tumor bearing nude mice.

中文翻译：

使用液相色谱-串联质谱法定量检测组织中聚合物微针递送的HA15。

开发了一种快速灵敏的液相色谱-串联质谱方法，并已用于测定HA15的含量，HA15是一种可溶的聚合物微针递送的靶向GSPA5 / BIP的新兴抗癌化合物。血浆和组织匀浆中HA15的定量线性范围为2.5-1000 ng / ml，检测限和定量下限分别为1和2.5 ng / ml。日间和日内准确性和精确度都在可接受的范围内。使用蛋白质沉淀并使用达布拉非尼作为内标从小鼠血浆和器官中提取HA15，该药物在相关分析条件下稳定。该方法用于分析药物载量，体外溶出度，

更新日期：2020-03-04

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