The purpose of this investigation is to evaluate the implementation of ultrasound-assisted liquid biphasic flotation (LBF) system for the recovery of natural astaxanthin from Haematococcus pluvialis microalgae. Various operating conditions of ultrasound-assisted LBF systems such as the position of ultrasound horn, mode of ultrasonication (pulse and continuous), amplitude of ultrasonication, air flowrate, duration of air flotation, and mass of H. pluvialis microalgae were evaluated. The effect of ultrasonication on the cellular morphology of microalgae was also assessed using microscopic analysis. Under the optimized operating conditions of UALBF, the maximum recovery yield, extraction efficiency, and partition coefficient of astaxanthin were 95.08 ± 3.02%, 99.74 ± 0.05%, and 185.09 ± 4.78, respectively. In addition, the successful scale-up operation of ultrasound-assisted LBF system verified the practicability of this integrated approach for an effective extraction of natural astaxanthin.

中文翻译：

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集成的超声辅助液体双相浮选法可从雨生红球菌中高效提取虾青素。

这项研究的目的是评估超声辅助液体双相浮选（LBF）系统的实施，以从红血球菌微藻中回收天然虾青素。评估了超声辅助LBF系统的各种工作条件，例如超声喇叭的位置，超声模式（脉冲和连续），超声幅度，空气流速，气浮持续时间以及雨生黄杆菌微藻的质量。还使用显微镜分析评估了超声处理对微藻细胞形态的影响。在UALBF的最佳操作条件下，虾青素的最大回收率，提取效率和分配系数分别为95.08±3.02％，99.74±0.05％和185.09±4.78。此外，