The effect of chemical refining process on the bioactive composition, in vitro antioxidant capacity, and their correlation of perilla seed oil (PSO) were investigated. In this paper, seven samples corresponding to each step of the refining process (degumming, neutralization, bleaching, deodorization, winterization, crude, and refined oils) were studied. The results showed that phenolic compounds and tocopherols were removed from PSO to a degree of 19.4% and 5.4%, respectively. In addition, the carotenoid content of PSO decreased during the refining process. The main carotenoid of PSO was found to be lutein, and the compound was lost completely during the bleaching step of the refining process. In this paper, we analyzed the variation of carotenoid content in PSO during the refining process for the first time. Neutralization affected the contents of phytosterols the most, followed by the effects of degumming and bleaching. The demonstrated results of Pearson product-moment correlation indicated that total tocopherols were significantly correlated with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorption capacity (ORAC) values, whereas carotenoids were significantly correlated with the DPPH value. However, phenolic compounds and phytosterols have no significant difference with DPPH, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ORAC, and ferric reducing antioxidant power values. The collected information can be applied to seeking out optimum factors needed to suffice the fundamental requirements for PSO production and minimize micronutrient losses to enhance its market value. PRACTICAL APPLICATION: The present study aimed to determine influence of chemical refining in the bioactive composition of perilla seed oil (PSO) as well as its antioxidant capacity in vitro. Moreover, we also intend to find the correlation between them. Results indicated that this study supplies a good reference for the industrial parameters of the refining process to minimize micronutrient losses and further obtain high-quality PSO products for consumers.

中文翻译：

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精炼工艺对紫苏籽油生物活性成分、体外抗氧化能力及其相关性的影响

研究了化学精炼工艺对紫苏籽油（PSO）的生物活性成分、体外抗氧化能力及其相关性的影响。在本文中，研究了对应于精炼过程（脱胶、中和、漂白、除臭、冬化、原油和精炼油）每个步骤的七个样品。结果表明，酚类化合物和生育酚从 PSO 中去除的程度分别为 19.4% 和 5.4%。此外，PSO 的类胡萝卜素含量在精炼过程中下降。发现 PSO 的主要类胡萝卜素是叶黄素，该化合物在精炼过程的漂白步骤中完全丢失。在本文中，我们首次分析了精制过程中 PSO 中类胡萝卜素含量的变化。中和作用对植物甾醇含量的影响最大，其次是脱胶和漂白作用。Pearson 积矩相关性的证明结果表明，总生育酚与 2,2-二苯基-1-苦基肼 (DPPH) 和氧自由基吸收能力 (ORAC) 值显着相关，而类胡萝卜素与 DPPH 值显着相关。然而，酚类化合物和植物甾醇与 DPPH、2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 二铵盐、ORAC 和三价铁的抗氧化能力值没有显着差异。收集到的信息可用于寻找满足 PSO 生产基本要求所需的最佳因素，并最大限度地减少微量营养素损失以提高其市场价值。实际应用：本研究旨在确定化学精制对紫苏籽油 (PSO) 生物活性成分的影响及其体外抗氧化能力。此外，我们还打算找出它们之间的相关性。结果表明，本研究为炼油工艺的工业参数提供了很好的参考，以最大限度地减少微量营养素的损失，进一步为消费者提供优质的PSO产品。