The photoperiod/temperature‐sensitive genic male sterile (P/TGMS) character is important for use of heterosis in hybrid wheat. In the present study, the fertility of 172 doubled haploid (DH) lines derived from the cross between a P/TGMS wheat line BS366 and a restorer wheat line Baiyu149 was investigated under both fertile and sterile environments during three cropping seasons. The phenotype was recorded based on the international seed setting rate. A total of 167 SSR and 1,278 SNP markers were used to construct a linkage map, with a total length of 3,748.94 cM and an average marker interval of 2.59 cM. Three QTLs were identified and designated as QF.bhw‐2DS, QF.bhw‐4BS and QF.bhw‐7Al, explaining 6.9%–12.8%, 19.7%–25.6% and 7.2%–8.8% of the phenotypic variances, respectively. These results lay a good basis for application of male sterility‐related molecular markers in improvement of two‐line hybrid wheat breeding system.

中文翻译：

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光周期和温度敏感型雄性不育小麦品系BS366雄性不育相关性状的QTL定位

光周期/温度敏感的基因雄性不育（P / TGMS）特性对于杂种小麦杂种优势的利用非常重要。在本研究中，在三个种植季节中，在肥沃和无菌环境下，研究了172个来自P / TGMS小麦系BS366和恢复小麦系Baiyu149杂交的双倍单倍体（DH）的育性。根据国际种子结实率记录表型。总共167个SSR和1,278个SNP标记用于构建连锁图谱，全长3,748.94 cM，平均标记间隔为2.59 cM。确定了三个QTL，并将其命名为QF.bhw-2DS，QF.bhw-4BS和QF.bhw-7Al，分别解释了表型差异的6.9％–12.8％，19.7％–25.6％和7.2％–8.8％。这些结果为将雄性不育相关分子标记应用于改良两系杂交小麦育种体系奠定了良好的基础。