当前位置: X-MOL 学术Nat. Plants › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Structure of a minimal photosystem I from the green alga Dunaliella salina.
Nature Plants ( IF 15.8 ) Pub Date : 2020-03-02 , DOI: 10.1038/s41477-020-0611-9
Annemarie Perez-Boerema 1, 2 , Daniel Klaiman 3 , Ido Caspy 3 , Sigal Y Netzer-El 3 , Alexey Amunts 1, 2 , Nathan Nelson 3
Affiliation  

Solar energy harnessed by oxygenic photosynthesis supports most of the life forms on Earth. In eukaryotes, photosynthesis occurs in chloroplasts and is achieved by membrane-embedded macromolecular complexes that contain core and peripheral antennae with multiple pigments. The structure of photosystem I (PSI) comprises the core and light-harvesting (LHCI) complexes, which together form PSI-LHCI. Here we determined the structure of PSI-LHCI from the salt-tolerant green alga Dunaliella salina using X-ray crystallography and electron cryo-microscopy. Our results reveal a previously undescribed configuration of the PSI core. It is composed of only 7 subunits, compared with 14-16 subunits in plants and the alga Chlamydomonas reinhardtii, and forms the smallest known PSI. The LHCI is poorly conserved at the sequence level and binds to pigments that form new energy pathways, and the interactions between the individual Lhca1-4 proteins are weakened. Overall, the data indicate the PSI of D. salina represents a different type of the molecular organization that provides important information for reconstructing the plasticity and evolution of PSI.

中文翻译:

来自绿藻杜氏盐藻的最小光系统I的结构。

含氧光合作用所利用的太阳能支持地球上大多数生命形式。在真核生物中,光合作用发生在叶绿体中,并且是通过膜嵌入的大分子复合物实现的,该复合物包含具有多种色素的核心和外围触角。光系统I(PSI)的结构包含核心和聚光(LHCI)配合物,它们共同形成PSI-LHCI。在这里,我们使用X射线晶体学和电子冷冻显微镜从耐盐绿藻杜氏盐藻中确定了PSI-LHCI的结构。我们的结果揭示了先前未描述的PSI内核配置。它仅由7个亚基组成,而在植物和莱茵衣藻中则为14-16个亚基,形成已知的最小PSI。LHCI在序列水平上保守性很差,并与形成新能量途径的色素结合,从而削弱了各个Lhca1-4蛋白之间的相互作用。总体而言,数据表明盐假单胞菌的PSI代表了不同类型的分子组织,为重建PSI的可塑性和进化提供了重要信息。
更新日期:2020-03-02
down
wechat
bug