Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo,Nature Methods

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Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
Nature Methods ( IF 36.1 ) Pub Date : 2020-03-02 , DOI: 10.1038/s41592-020-0762-7
Jianglai Wu _{1,

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3} , Yajie Liang ₃ , Shuo Chen ₁ , Ching-Lung Hsu ₃ , Mariya Chavarha ₄ , Stephen W Evans ₄ , Dongqing Shi ₄ , Michael Z Lin ₄ , Kevin K Tsia ₂ , Na Ji _{1,

3,

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Affiliation

Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.

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