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Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution.
Nature Cell Biology ( IF 17.3 ) Pub Date : 2020-03-02 , DOI: 10.1038/s41556-020-0475-2 Yanzhu Yue 1 , Weijian Zong 1 , Xin Li 1 , Jinghang Li 1 , Youdong Zhang 1 , Runlong Wu 1, 2 , Yazui Liu 3 , Jiahao Cui 3 , Qianhao Wang 1 , Yunkun Bian 1 , Xianhong Yu 1 , Yao Liu 4 , Guangming Tan 4 , Yunfeng Zhang 2 , Gang Zhao 3 , Bin Zhou 5 , Liangyi Chen 1 , Wenlei Xiao 3 , Heping Cheng 1 , Aibin He 1
Nature Cell Biology ( IF 17.3 ) Pub Date : 2020-03-02 , DOI: 10.1038/s41556-020-0475-2 Yanzhu Yue 1 , Weijian Zong 1 , Xin Li 1 , Jinghang Li 1 , Youdong Zhang 1 , Runlong Wu 1, 2 , Yazui Liu 3 , Jiahao Cui 3 , Qianhao Wang 1 , Yunkun Bian 1 , Xianhong Yu 1 , Yao Liu 4 , Guangming Tan 4 , Yunfeng Zhang 2 , Gang Zhao 3 , Bin Zhou 5 , Liangyi Chen 1 , Wenlei Xiao 3 , Heping Cheng 1 , Aibin He 1
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Mapping of the holistic cell behaviours sculpting the four-chambered mammalian heart has been a goal or previous studies, but so far only success in transparent invertebrates and lower vertebrates with two-chambered hearts has been achieved. Using a live-imaging system comprising a customized vertical light-sheet microscope equipped with a mouse embryo culture module, a heartbeat-gated imaging strategy and a digital image processing framework, we realized volumetric imaging of developing mouse hearts at single-cell resolution and with uninterrupted cell lineages for up to 1.5 d. Four-dimensional landscapes of Nppa+ cardiomyocyte cell behaviours revealed a blueprint for ventricle chamber formation by which biased outward migration of the outermost cardiomyocytes is coupled with cell intercalation and horizontal division. The inner-muscle architecture of trabeculae was developed through dual mechanisms: early fate segregation and transmural cell arrangement involving both oriented cell division and directional migration. Thus, live-imaging reconstruction of uninterrupted cell lineages affords a transformative means for deciphering mammalian organogenesis.
中文翻译：
在单细胞分辨率下对小鼠心室腔形成过程中心肌细胞行为的长期，实时成像。
雕刻四腔哺乳动物心脏的整体细胞行为图谱是一个目标或以前的研究，但到目前为止，仅在透明无脊椎动物和具有两腔心脏的低等脊椎动物中取得了成功。通过使用实时成像系统，该系统包括配备有小鼠胚胎培养模块的定制垂直光片显微镜，心跳门控成像策略和数字图像处理框架，我们实现了以单细胞分辨率并以不间断的细胞谱系长达1.5 d。Nppa +心肌细胞行为的四维景观揭示了心室形成的蓝图，通过该蓝图，最外层心肌细胞的偏向向外迁移与细胞嵌入和水平分裂相结合。小梁的内部肌肉结构是通过双重机制开发的：早期命运分离和涉及定向细胞分裂和定向迁移的透壁细胞排列。因此，不间断细胞谱系的实时成像重建为解密哺乳动物器官发生提供了一种转化手段。
更新日期：2020-04-24
中文翻译：
在单细胞分辨率下对小鼠心室腔形成过程中心肌细胞行为的长期，实时成像。
雕刻四腔哺乳动物心脏的整体细胞行为图谱是一个目标或以前的研究，但到目前为止，仅在透明无脊椎动物和具有两腔心脏的低等脊椎动物中取得了成功。通过使用实时成像系统，该系统包括配备有小鼠胚胎培养模块的定制垂直光片显微镜，心跳门控成像策略和数字图像处理框架，我们实现了以单细胞分辨率并以不间断的细胞谱系长达1.5 d。Nppa +心肌细胞行为的四维景观揭示了心室形成的蓝图，通过该蓝图，最外层心肌细胞的偏向向外迁移与细胞嵌入和水平分裂相结合。小梁的内部肌肉结构是通过双重机制开发的：早期命运分离和涉及定向细胞分裂和定向迁移的透壁细胞排列。因此，不间断细胞谱系的实时成像重建为解密哺乳动物器官发生提供了一种转化手段。
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