The TaMP gene from wheat encodes an α-mannosidase induced by salt stress that functions as negative regulator of salt tolerance in plants. Salt stress significantly affects growth and yield of crop plants. The α-mannosidases function in protein folding, trafficking, and endoplasmic reticulum-associated degradation in eukaryotic cells, and they are involved in abiotic stress tolerance in plants. Previously, we identified the α-mannosidase gene TaMP in wheat (Triticum aestivum). In this study, we investigated the function of TaMP in salt stress tolerance. TaMP expression was induced in wheat leaves by salt, drought, abscisic acid, and H2O2 treatments. Overexpressing TaMP in Brachypodium distachyon was associated with a salt-sensitive phenotype. Under salt stress, the overexpressing plants had reduced height, delayed growth status, low photosynthetic rate, decreased survival rate, and diminished yield. Moreover, the overexpression of TaMP aggravated the tendency for ions to become toxic under salt stress by significantly affecting the Na+ and K+ contents in cells. In addition, TaMP could negatively regulate salt tolerance by affecting the antioxidant enzyme system capacity and increasing the reactive oxygen species accumulation. Our study was helpful to understand the underlying physiological and molecular mechanisms of salt stress tolerance in plants.

中文翻译：

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小麦α-甘露糖苷酶基因TaMP的过表达损害转基因二穗短柄草的耐盐性。

来自小麦的 TaMP 基因编码一种由盐胁迫诱导的 α-甘露糖苷酶，其作为植物耐盐性的负调节剂。盐胁迫显着影响农作物的生长和产量。α-甘露糖苷酶在真核细胞中的蛋白质折叠、运输和内质网相关降解中发挥作用，并且它们参与植物的非生物胁迫耐受性。此前，我们在小麦 (Triticum aestivum) 中鉴定了 α-甘露糖苷酶基因 TaMP。在这项研究中，我们研究了 TaMP 在耐盐胁迫中的作用。盐、干旱、脱落酸和 H2O2 处理可诱导小麦叶片中的 TaMP 表达。在 Brachypodium distachyon 中过表达 TaMP 与盐敏感表型有关。在盐胁迫下，过度表达的植物高度降低，生长状态延迟，光合速率低，成活率下降，产量下降。此外，TaMP的过表达通过显着影响细胞中Na+和K+的含量，加剧了盐胁迫下离子毒性的趋势。此外，TaMP 可以通过影响抗氧化酶系统的能力和增加活性氧的积累来负调节盐耐受性。我们的研究有助于了解植物耐盐胁迫的潜在生理和分子机制。TaMP可以通过影响抗氧化酶系统的能力和增加活性氧的积累来负调节盐耐受性。我们的研究有助于了解植物耐盐胁迫的潜在生理和分子机制。TaMP可以通过影响抗氧化酶系统的能力和增加活性氧的积累来负调节盐耐受性。我们的研究有助于了解植物耐盐胁迫的潜在生理和分子机制。