2,5-Furandicarboxylic acid (FDCA) is a promising bio-based building block as a green alternative to petroleum-based terephthalate in polymer production. Most of FDCA is produced by the oxidation of 5-hydroxymethylfurfural (HMF), which is derived from hexose. Although the chemical conversion is widely applied, the biocatalytic conversion is expected due to the relatively mild condition and fewer toxic chemicals consumption. However, it’s difficult to catalyze the conversion of HMF to FDCA by a single enzyme. Here, a newly enzymatic cascade reaction process was introduced with a yield of 94.0% by the combination of 5-hydroxymethylfurfural oxidase (HMFO) and lipase. Briefly, a flavine adenosine dinucleotide independent (FAD-independent) HMFO of Methylovorus sp. MP688 was used to convert HMF to 2,5-diformylfuran (DFF) and 5-formylfuroic acid (FFA), which consecutively transformed to FDCA by a lipase Novozym 435. To facilitate the purification, a coupled alkali precipitation was developed to recover FDCA from organic solvent with an improved purity from 84.4 to 99.0% and recovery of 78.1%. This work will help to construct the green biorefinery route for the bulk FDCA from biomass by enzymes.

2,5-呋喃二甲酸（FDCA）是一种有前途的生物基构件，可作为聚合物生产中石油基对苯二甲酸酯的绿色替代品。大部分FDCA都是由5-羟甲基糠醛（HMF）氧化生成的，而HMF是由己糖衍生而来的。尽管化学转化得到广泛应用，但是由于条件相对温和且毒性化学药品消耗较少，因此有望实现生物催化转化。但是，很难通过单一酶催化HMF向FDCA的转化。在这里，通过5-羟甲基糠醛氧化酶（HMFO）和脂肪酶的组合，引入了一种新的酶促级联反应工艺，产率为94.0％。简而言之，甲伏牛的黄素腺苷二核苷酸非依赖性（非FAD依赖性）HMFOsp。MP688用于将HMF转化为2,5-二甲酰呋喃（DFF）和5-甲酰糠酸（FFA），后者通过脂肪酶Novozym 435连续转化为FDCA。为促进纯化，开发了碱耦合沉淀法以从中回收FDCA。有机溶剂，纯度从84.4％提高到99.0％，回收率达到78.1％。这项工作将有助于通过酶从生物质中构建散装FDCA的绿色生物精炼路线。