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MoS2 nanostructured materials for electrode modification in the development of a laccase based amperometric biosensor for non-invasive dopamine detection
Microchemical Journal ( IF 4.9 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.microc.2020.104792
Rodrigo Rubio-Govea , David P. Hickey , Raúl García-Morales , Melissa Rodriguez-Delgado , Mario A. Domínguez-Rovira , Shelley D. Minteer , Nancy Ornelas-Soto , Alejandra García-García

Abstract A novel laccase-based amperometric biosensor was developed through the modification of carbon paper electrodes with layered two-dimensional molybdenum disulfide (MoS2) presented in two different morphologies, flowers (MoS2-F) and ribbons (MoS2-R). Two laccase isoforms from Pycnoporus sanguineus CS43 fungi (LacI and LacII) were evaluated for the first time as a biological recognition element, and their performance was compared with commercial laccase from Trametes versicolor (TvL). The best results were observed with the MoS2-R morphology at a concentration of 2 mg/mL Biosensors modified with MoS2-R and with the three enzymes, showed two linear ranges of detection, from 0.1 to 0.5 µM and from 1 to 5 µM. Biosensors modified with LacII exhibited a high sensitivity of 340.3 nA/µM; and low limit of detection (LOD) of 10 nM, which are the best values reported in the literature for dopamine detection by laccase based biosensor. Optimized electrodes modified with enzymes LacII and TvL were tested in a synthetic urine sample in order to evaluate their practical effectiveness, resulting in LOD of 0.67 and 2.67 µM respectively, which are below the average concentrations of dopamine normally found in real urine samples (around 2 µM).

中文翻译:

MoS2 纳米结构材料用于电极修饰,用于开发基于漆酶的安培生物传感器,用于非侵入性多巴胺检测

摘要 通过使用呈现两种不同形态的层状二维二硫化钼 (MoS2) 修饰碳纸电极,开发了一种新型基于漆酶的电流型生物传感器,该电极具有花 (MoS2-F) 和带状 (MoS2-R) 两种形态。首次评估了来自 Pycnoporus sanguineus CS43 真菌(LacI 和 LacII)的两种漆酶同工型作为生物识别元件,并将它们的性能与来自变色栓菌 (TvL) 的商业漆酶进行了比较。最好的结果是在浓度为 2 mg/mL 的 MoS2-R 形态下观察到的。 用 MoS2-R 和三种酶修饰的生物传感器显示出两个线性检测范围,从 0.1 到 0.5 µM 和从 1 到 5 µM。用 LacII 修饰的生物传感器表现出 340.3 nA/µM 的高灵敏度;检测下限 (LOD) 为 10 nM,这是文献中报道的通过基于漆酶的生物传感器检测多巴胺的最佳值。用酶 LacII 和 TvL 修饰的优化电极在合成尿样中进行了测试,以评估其实际有效性,结果 LOD 分别为 0.67 和 2.67 µM,低于通常在真实尿样中发现的多巴胺平均浓度(约 2微米)。
更新日期:2020-06-01
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