The excessive use of chemical fertilizers in conventional agricultural systems decreased the nutrient use efficiency and caused serious environmental problems such as waterway pollution, mineral depletion, soil acidification and other issues. In order to achieve the desirable essential oil productivity and reduction consumption of chemical inputs in peppermint (Mentha x piperita L.), a 2-year field experiment was carried out using a split-plot approach based on a randomized complete block design (RCBD), with 7 treatments and three replications at two harvesting times. The main factor was given by different fertilizer treatments including no fertilizer (control), chemical fertilizer, arbuscular mycorrhiza fungus, 50 % chemical fertilizer + arbuscular mycorrhiza fungus, nano chelated fertilizer, 50 % chemical fertilizer + nano chelated fertilizer, nano chelated fertilizer + arbuscular mycorrhiza fungus, and the sub-factor included two harvesting times (first harvest and second harvest). The results demonstrated that the highest and lowest growth parameters including plant height, number of lateral branches per plant and leaf greenness (SPAD index) were achieved with integrative application of 50 % chemical fertilizer + nano chelated fertilizer (in the first harvest) and control conditions (in the second harvest), respectively. Also, the maximum concentration of N, P, K and Fe was reached in the first harvest with application of 50 % chemical fertilizer + nano chelated fertilizer. Furthermore, the highest peppermint dry matter yield (354.8 g/m²), essential oil content (2.7 %) and essential oil yield (6.6 g/m²) was achieved at the first harvest with application of 50 % chemical fertilizer + nano chelated fertilizer. GC–MS analysis of peppermint essential oil showed that the major components at first harvest were menthol (31.82–37.87 %), menthone (23.85–30.90 %), 1,8-cineole (6.39–6.82 %), δ-terpineol (3.61–4.11 %) and neo-menthol (2.67–3.33 %), whereas at second harvest menthol (44–47.31 %), p-menth-l-en-9-ol (11.66–14.96 %), menthofuran (3.44–5.14 %), menthone (3.82–10.62 %), 1,8-cineole (5.51–5.99 %) and neo-menthol (5.03–5.90 %). Notably, menthol reached the highest amount with application of 50 % chemical fertilizer + nano chelated fertilizer. Overall, an integrative application of chemical fertilizers with nano fertilizers can be suggested to farmers as an alternative and environmentally friendly strategy to improve the quali-quantitative characteristics of peppermint essential oil.

传统农业系统过度使用化肥会降低养分的利用效率，并造成严重的环境问题，例如水路污染，矿物质消耗，土壤酸化等问题。为了达到理想的精油生产率并减少薄荷中化学原料的消耗（薄荷油（Mentha x piperita）L.），使用基于随机完全区组设计（RCBD）的分割图方法进行了为期2年的田间试验，在两次收获时间进行了7种处理和3次重复。主要因素由不同的肥料处理方式决定，包括不施肥（对照），化肥，丛枝菌根真菌，50％的化肥+丛枝菌根真菌，纳米螯合肥料，50％的化肥+纳米螯合肥料，纳米螯合肥料+丛枝肥料菌根真菌和次要因素包括两次收获时间（第一次收获和第二次收获）。结果表明，最高和最低生长参数包括株高，通过分别施用50％化肥+纳米螯合肥料（第一次收获）和控制条件（第二次收获）分别获得了每株植物的侧枝数量和叶片绿色度（SPAD指数）。此外，在首次收获时，施用50％化学肥料+纳米螯合肥料达到了N，P，K和Fe的最大浓度。此外，薄荷干物质产量最高（354.8 g / m²），在第一次收获时，施用50％的化学肥料+纳米螯合肥料可达到精油含量（2.7％）和精油产量（6.6 g / m ²）。薄荷精油的GC-MS分析表明，初榨时的主要成分为薄荷醇（31.82–37.87％），薄荷酮（23.85–30.90％），1,8-桉树脑（6.39–6.82％），δ-松油醇（3.61 -4.11％）和新-薄荷醇（2.67-3.33％），而在第二收获薄荷醇（44-47.31％），p -menth-升烯-9-醇（11.66-14.96％），薄荷呋喃（3.44-5.14 ％），薄荷酮（3.82–10.62％），1,8-桉树脑（5.51–5.99％）和新-薄荷醇（5.03-5.90％）。值得注意的是，薄荷醇在施用50％化学肥料+纳米螯合肥料时达到最高含量。总体而言，可以向农民建议将化肥与纳米肥料综合应用，作为改善薄荷精油定性定量特性的替代性和环境友好策略。